Comparison of the Effects of Mecillinam and 6-Aminopenicillanic Acid on Proteus mirabilis, Escherichia coli, and Staphylococcus aureus

Single strains of Proteus mirabilis, Escherichia coli, and Staphylococcus aureus were grown on filter membranes placed on agar containing concentration series of mecillinam (FL 1060), 6-aminopenicillanic acid (6-APA), or ampicillin. P. mirabilis and E. coli were also exposed to combinations of mecil...

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Main Authors: Lorian, Victor, Atkinson, Barbara
Formato: Artigo
Idioma:en
Publicado em: 1977
Assuntos:
Biosynthesis; Chemistry; Mechanisms of Action and Resistance
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC352018/
https://ncbi.nlm.nih.gov/pubmed/193439
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spelling pubmed-3520182004-07-12 Comparison of the Effects of Mecillinam and 6-Aminopenicillanic Acid on Proteus mirabilis, Escherichia coli, and Staphylococcus aureus Lorian, Victor Atkinson, Barbara Antimicrob Agents Chemother Biosynthesis; Chemistry; Mechanisms of Action and Resistance Single strains of Proteus mirabilis, Escherichia coli, and Staphylococcus aureus were grown on filter membranes placed on agar containing concentration series of mecillinam (FL 1060), 6-aminopenicillanic acid (6-APA), or ampicillin. P. mirabilis and E. coli were also exposed to combinations of mecillinam or 6-APA with ampicillin. Colony-forming units were counted, and cells were examined by interference phase-contrast and transmission electron microscopy. Mecillinam and 6-APA were very effective in reducing the viability of the two gram-negative species, but they were less effective against S. aureus. Combinations of mecillinam and 6-APA with ampicillin acted synergistically against the gram-negative bacilli. When the antibiotics were presented consecutively, their effects on viability were usually no greater than the effects of the individual antibiotics acting alone. When P. mirabilis and E. coli were exposed to mecillinam alone or in combination with ampicillin, the cells became rounded. 6-APA alone or in combination with ampicillin produced elongated polymorphic cells in these species. The most unusual morphological effects were ultrastructural. Mecillinam and, to a lesser extent, 6-APA produced inward growth of numerous pairs of trilamellar membranous structures within the cells. It is possible that these membranes represent the growth initiation of aberrant cross walls. Both mecillinam and 6-APA produced multiple, thick cross walls in S. aureus. 1977-03 /pmc/articles/PMC352018/ /pubmed/193439 Text en Copyright © 1977 American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Biosynthesis; Chemistry; Mechanisms of Action and Resistance
spellingShingle Biosynthesis; Chemistry; Mechanisms of Action and Resistance
Lorian, Victor
Atkinson, Barbara
Comparison of the Effects of Mecillinam and 6-Aminopenicillanic Acid on Proteus mirabilis, Escherichia coli, and Staphylococcus aureus
description Single strains of Proteus mirabilis, Escherichia coli, and Staphylococcus aureus were grown on filter membranes placed on agar containing concentration series of mecillinam (FL 1060), 6-aminopenicillanic acid (6-APA), or ampicillin. P. mirabilis and E. coli were also exposed to combinations of mecillinam or 6-APA with ampicillin. Colony-forming units were counted, and cells were examined by interference phase-contrast and transmission electron microscopy. Mecillinam and 6-APA were very effective in reducing the viability of the two gram-negative species, but they were less effective against S. aureus. Combinations of mecillinam and 6-APA with ampicillin acted synergistically against the gram-negative bacilli. When the antibiotics were presented consecutively, their effects on viability were usually no greater than the effects of the individual antibiotics acting alone. When P. mirabilis and E. coli were exposed to mecillinam alone or in combination with ampicillin, the cells became rounded. 6-APA alone or in combination with ampicillin produced elongated polymorphic cells in these species. The most unusual morphological effects were ultrastructural. Mecillinam and, to a lesser extent, 6-APA produced inward growth of numerous pairs of trilamellar membranous structures within the cells. It is possible that these membranes represent the growth initiation of aberrant cross walls. Both mecillinam and 6-APA produced multiple, thick cross walls in S. aureus.
author Lorian, Victor
Atkinson, Barbara
author_facet Lorian, Victor
Atkinson, Barbara
author_sort Lorian, Victor
title Comparison of the Effects of Mecillinam and 6-Aminopenicillanic Acid on Proteus mirabilis, Escherichia coli, and Staphylococcus aureus
title_short Comparison of the Effects of Mecillinam and 6-Aminopenicillanic Acid on Proteus mirabilis, Escherichia coli, and Staphylococcus aureus
title_full Comparison of the Effects of Mecillinam and 6-Aminopenicillanic Acid on Proteus mirabilis, Escherichia coli, and Staphylococcus aureus
title_fullStr Comparison of the Effects of Mecillinam and 6-Aminopenicillanic Acid on Proteus mirabilis, Escherichia coli, and Staphylococcus aureus
title_full_unstemmed Comparison of the Effects of Mecillinam and 6-Aminopenicillanic Acid on Proteus mirabilis, Escherichia coli, and Staphylococcus aureus
title_sort comparison of the effects of mecillinam and 6-aminopenicillanic acid on proteus mirabilis, escherichia coli, and staphylococcus aureus
publishDate 1977
url https://ncbi.nlm.nih.gov/pmc/articles/PMC352018/
https://ncbi.nlm.nih.gov/pubmed/193439
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