Structural components of mouse mammary tumor virus. II. Isolation and purification of virion polypeptides.

Mouse mammary tumor virus (MMTV) glycoproteins and nonglycosylated polypeptides were purified by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Primary amino groups were labeled with fluorescamine to enable visualization of MMTV polypeptides in the gels. Protein bands were sl...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Yagi, M J, Stutzman, R E, Robertson, B H, Compans, R W
Formato: Artigo
Idioma:en
Publicado em: 1978
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC354082/
https://ncbi.nlm.nih.gov/pubmed/207897
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
id pubmed-354082
record_format dspace
spelling pubmed-3540822004-03-04 Structural components of mouse mammary tumor virus. II. Isolation and purification of virion polypeptides. Yagi, M J Stutzman, R E Robertson, B H Compans, R W J Virol Research Article Mouse mammary tumor virus (MMTV) glycoproteins and nonglycosylated polypeptides were purified by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Primary amino groups were labeled with fluorescamine to enable visualization of MMTV polypeptides in the gels. Protein bands were sliced from the gels and eluted with 90 to 95% recovery. Eight MMTV polypeptides, including three of the major viral components as well as five minor proteins, were routinely obtained. Double diffusion assays and immunoelectrophoresis confirmed the retention of antigenicity identical to that of untreated MMTV virions. Antisera obtained from MMTV-free BALB/c mice immunized with these purified proteins reacted with the polypeptide immunogen as well as with detergent-disrupted MMTV virions from mouse milk or cell culture. Double diffusion assays using the specific mouse antisera failed to detect any cross-reactivity among the isolated polypeptides. A hemagglutination-inhibition assay demonstrated that the ability of MMTV virions to inhibit the hemagglutinating properties of influenza virus resides in the glycosylated polypeptides gp52, gp37.7, and gp33. 1978-05 /pmc/articles/PMC354082/ /pubmed/207897 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Yagi, M J
Stutzman, R E
Robertson, B H
Compans, R W
Structural components of mouse mammary tumor virus. II. Isolation and purification of virion polypeptides.
description Mouse mammary tumor virus (MMTV) glycoproteins and nonglycosylated polypeptides were purified by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Primary amino groups were labeled with fluorescamine to enable visualization of MMTV polypeptides in the gels. Protein bands were sliced from the gels and eluted with 90 to 95% recovery. Eight MMTV polypeptides, including three of the major viral components as well as five minor proteins, were routinely obtained. Double diffusion assays and immunoelectrophoresis confirmed the retention of antigenicity identical to that of untreated MMTV virions. Antisera obtained from MMTV-free BALB/c mice immunized with these purified proteins reacted with the polypeptide immunogen as well as with detergent-disrupted MMTV virions from mouse milk or cell culture. Double diffusion assays using the specific mouse antisera failed to detect any cross-reactivity among the isolated polypeptides. A hemagglutination-inhibition assay demonstrated that the ability of MMTV virions to inhibit the hemagglutinating properties of influenza virus resides in the glycosylated polypeptides gp52, gp37.7, and gp33.
author Yagi, M J
Stutzman, R E
Robertson, B H
Compans, R W
author_facet Yagi, M J
Stutzman, R E
Robertson, B H
Compans, R W
author_sort Yagi, M J
title Structural components of mouse mammary tumor virus. II. Isolation and purification of virion polypeptides.
title_short Structural components of mouse mammary tumor virus. II. Isolation and purification of virion polypeptides.
title_full Structural components of mouse mammary tumor virus. II. Isolation and purification of virion polypeptides.
title_fullStr Structural components of mouse mammary tumor virus. II. Isolation and purification of virion polypeptides.
title_full_unstemmed Structural components of mouse mammary tumor virus. II. Isolation and purification of virion polypeptides.
title_sort structural components of mouse mammary tumor virus. ii. isolation and purification of virion polypeptides.
publishDate 1978
url https://ncbi.nlm.nih.gov/pmc/articles/PMC354082/
https://ncbi.nlm.nih.gov/pubmed/207897
_version_ 1759382394092126208

Registos relacionados