Intracytoplasmic A Particles: Mouse Mammary Tumor Virus Nucleoprotein Cores?

A rapid method for the isolation of intracytoplasmic A particles, the putative intracellular nucleoprotein cores of mouse mammary tumor virus (MTV), is presented. Spontaneous C3H/He mouse mammary tumors and transplantable mouse Leydig cell tumor were used as source material. Large aggregations of in...

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Hlavní autoři: Smith, Gilbert H., Wivel, Nelson A.
Médium: Článek
Jazyk:en
Vydáno: 1973
Témata:
Animal Viruses
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC355139/
https://ncbi.nlm.nih.gov/pubmed/4349496
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spelling pubmed-3551392004-03-04 Intracytoplasmic A Particles: Mouse Mammary Tumor Virus Nucleoprotein Cores? Smith, Gilbert H. Wivel, Nelson A. J Virol Animal Viruses A rapid method for the isolation of intracytoplasmic A particles, the putative intracellular nucleoprotein cores of mouse mammary tumor virus (MTV), is presented. Spontaneous C3H/He mouse mammary tumors and transplantable mouse Leydig cell tumor were used as source material. Large aggregations of intracytoplasmic A particles were separated from cellular contaminants on discontinuous sucrose gradients and subsequently further purified by isopycnic banding in linear sucrose gradients. The purified particles were solubilized in sodium dodecyl sulfate, and the structural proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Purified mouse mammary tumor virions were solubilized, and their proteins were analyzed in the same way. Comparison of co-electrophoretic gels indicated a lack of correlation in the molecular size of the major structural proteins in intracytoplasmic A particles and MTV. The three major proteins of the A particles were calculated to be 80,000; 35,000; and 20,000 daltons. Five major polyacrylamide gel electrophoresis bands were obtained with purified MTV; these were 90,000; 69,000; 55,000; 37,000; and 27,500 daltons. These figures showed good correlation with those published for MTV by Nowinski et al. These results suggest the need for the reexamination of the current tenet that intracytoplasmic particles represent intracellular MTV nucleoprotein cores. 1973-04 /pmc/articles/PMC355139/ /pubmed/4349496 Text en Copyright © 1973 American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Animal Viruses
spellingShingle Animal Viruses
Smith, Gilbert H.
Wivel, Nelson A.
Intracytoplasmic A Particles: Mouse Mammary Tumor Virus Nucleoprotein Cores?
description A rapid method for the isolation of intracytoplasmic A particles, the putative intracellular nucleoprotein cores of mouse mammary tumor virus (MTV), is presented. Spontaneous C3H/He mouse mammary tumors and transplantable mouse Leydig cell tumor were used as source material. Large aggregations of intracytoplasmic A particles were separated from cellular contaminants on discontinuous sucrose gradients and subsequently further purified by isopycnic banding in linear sucrose gradients. The purified particles were solubilized in sodium dodecyl sulfate, and the structural proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Purified mouse mammary tumor virions were solubilized, and their proteins were analyzed in the same way. Comparison of co-electrophoretic gels indicated a lack of correlation in the molecular size of the major structural proteins in intracytoplasmic A particles and MTV. The three major proteins of the A particles were calculated to be 80,000; 35,000; and 20,000 daltons. Five major polyacrylamide gel electrophoresis bands were obtained with purified MTV; these were 90,000; 69,000; 55,000; 37,000; and 27,500 daltons. These figures showed good correlation with those published for MTV by Nowinski et al. These results suggest the need for the reexamination of the current tenet that intracytoplasmic particles represent intracellular MTV nucleoprotein cores.
author Smith, Gilbert H.
Wivel, Nelson A.
author_facet Smith, Gilbert H.
Wivel, Nelson A.
author_sort Smith, Gilbert H.
title Intracytoplasmic A Particles: Mouse Mammary Tumor Virus Nucleoprotein Cores?
title_short Intracytoplasmic A Particles: Mouse Mammary Tumor Virus Nucleoprotein Cores?
title_full Intracytoplasmic A Particles: Mouse Mammary Tumor Virus Nucleoprotein Cores?
title_fullStr Intracytoplasmic A Particles: Mouse Mammary Tumor Virus Nucleoprotein Cores?
title_full_unstemmed Intracytoplasmic A Particles: Mouse Mammary Tumor Virus Nucleoprotein Cores?
title_sort intracytoplasmic a particles: mouse mammary tumor virus nucleoprotein cores?
publishDate 1973
url https://ncbi.nlm.nih.gov/pmc/articles/PMC355139/
https://ncbi.nlm.nih.gov/pubmed/4349496
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