The rRNA enhancer regulates rRNA transcription in Saccharomyces cerevisiae.

In Saccharomyces cerevisiae, the rRNA genes are organized as a tandem array of head-to-tail repeats. An enhancer of rRNA transcription is present just at the end of each transcription unit, 2 kb away from the next one. This enhancer is unusual for S. cerevisiae in that it acts both upstream and down...

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Main Authors: Morrow, B E, Johnson, S P, Warner, J R
Format: Artigo
Sprog:en
Udgivet: 1993
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Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC359013/
https://ncbi.nlm.nih.gov/pubmed/8423793
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spelling pubmed-3590132004-03-11 The rRNA enhancer regulates rRNA transcription in Saccharomyces cerevisiae. Morrow, B E Johnson, S P Warner, J R Mol Cell Biol Research Article In Saccharomyces cerevisiae, the rRNA genes are organized as a tandem array of head-to-tail repeats. An enhancer of rRNA transcription is present just at the end of each transcription unit, 2 kb away from the next one. This enhancer is unusual for S. cerevisiae in that it acts both upstream and downstream of, and even across, genes. The role of the enhancer in the nutritional regulation of rRNA transcription was studied by introducing a centromere plasmid carrying two rRNA minigenes in tandem, flanking a single enhancer, into cells. Analysis of the transcripts from the two minigenes showed that the enhancer was absolutely required for the stimulation of transcription of rRNA that occurs when cells are shifted from a poor carbon source to a good carbon source. While full enhancer function is provided by a 45-bp region at the 3' end of the 190-bp enhancer, some activity was also conferred by other elements, including both a T-rich stretch and a region containing the binding sites for the proteins Reb1p and Abf1p. We conclude that the enhancer is composed of redundant elements and that it is a major element in the regulation of rRNA transcription. 1993-02 /pmc/articles/PMC359013/ /pubmed/8423793 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Morrow, B E
Johnson, S P
Warner, J R
The rRNA enhancer regulates rRNA transcription in Saccharomyces cerevisiae.
description In Saccharomyces cerevisiae, the rRNA genes are organized as a tandem array of head-to-tail repeats. An enhancer of rRNA transcription is present just at the end of each transcription unit, 2 kb away from the next one. This enhancer is unusual for S. cerevisiae in that it acts both upstream and downstream of, and even across, genes. The role of the enhancer in the nutritional regulation of rRNA transcription was studied by introducing a centromere plasmid carrying two rRNA minigenes in tandem, flanking a single enhancer, into cells. Analysis of the transcripts from the two minigenes showed that the enhancer was absolutely required for the stimulation of transcription of rRNA that occurs when cells are shifted from a poor carbon source to a good carbon source. While full enhancer function is provided by a 45-bp region at the 3' end of the 190-bp enhancer, some activity was also conferred by other elements, including both a T-rich stretch and a region containing the binding sites for the proteins Reb1p and Abf1p. We conclude that the enhancer is composed of redundant elements and that it is a major element in the regulation of rRNA transcription.
author Morrow, B E
Johnson, S P
Warner, J R
author_facet Morrow, B E
Johnson, S P
Warner, J R
author_sort Morrow, B E
title The rRNA enhancer regulates rRNA transcription in Saccharomyces cerevisiae.
title_short The rRNA enhancer regulates rRNA transcription in Saccharomyces cerevisiae.
title_full The rRNA enhancer regulates rRNA transcription in Saccharomyces cerevisiae.
title_fullStr The rRNA enhancer regulates rRNA transcription in Saccharomyces cerevisiae.
title_full_unstemmed The rRNA enhancer regulates rRNA transcription in Saccharomyces cerevisiae.
title_sort rrna enhancer regulates rrna transcription in saccharomyces cerevisiae.
publishDate 1993
url https://ncbi.nlm.nih.gov/pmc/articles/PMC359013/
https://ncbi.nlm.nih.gov/pubmed/8423793
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