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Purification and characterization of nitrile hydratase of mutant 4D of Rhodococcus rhodochrous PA-34
Nitrile hydratase (NHase; E.C. 4.2.1.84) has been purified and characterized using ammonium sulfate precipitation, ion exchange chromatography and gel filtration chromatography from the mutant 4D of Rhodococcus rhodochrous PA-34. The SDS-PAGE and MALDI-TOF analysis of the purified enzyme revealed th...
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| Format: | Article |
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| Idioma: | en |
| Publicat: |
Springer Berlin Heidelberg
2012
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| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3597139/ https://ncbi.nlm.nih.gov/pubmed/28324571 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-012-0081-5 |
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