Transformation of Tetrahymena thermophila with hypermethylated rRNA genes.

The extrachromosomal rRNA genes (rDNA) of Tetrahymena thermophila contain 0.4% N6-methyladenine. C3 strain rDNA was isolated, hypermethylated in vitro, and microinjected into B strain host cells. Clonal cell lines were established, and transformants were selected on the basis of resistance to paromo...

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Asıl Yazarlar: Karrer, K M, Yao, M C
Materyal Türü: Makale
Dil:en
Baskı/Yayın Bilgisi: 1988
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC363326/
https://ncbi.nlm.nih.gov/pubmed/3380092
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spelling pubmed-3633262004-03-11 Transformation of Tetrahymena thermophila with hypermethylated rRNA genes. Karrer, K M Yao, M C Mol Cell Biol Research Article The extrachromosomal rRNA genes (rDNA) of Tetrahymena thermophila contain 0.4% N6-methyladenine. C3 strain rDNA was isolated, hypermethylated in vitro, and microinjected into B strain host cells. Clonal cell lines were established, and transformants were selected on the basis of resistance to paromomycin, conferred by the injected rDNA. The effects of methylation by three enzymes which methylate the sequence 5'-NAT-3', the dam, EcoRI, and ClaI methylases, were tested. Hypermethylation of the injected rDNA had no effect on transformation efficiency relative to mock-methylated controls. The injected C3 strain rDNA efficiently replaced host rDNA as the major constituent of the population of rDNA molecules. Hypermethylation of the injected DNA was not maintained through 20 to 25 cell generations. 1988-04 /pmc/articles/PMC363326/ /pubmed/3380092 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Karrer, K M
Yao, M C
Transformation of Tetrahymena thermophila with hypermethylated rRNA genes.
description The extrachromosomal rRNA genes (rDNA) of Tetrahymena thermophila contain 0.4% N6-methyladenine. C3 strain rDNA was isolated, hypermethylated in vitro, and microinjected into B strain host cells. Clonal cell lines were established, and transformants were selected on the basis of resistance to paromomycin, conferred by the injected rDNA. The effects of methylation by three enzymes which methylate the sequence 5'-NAT-3', the dam, EcoRI, and ClaI methylases, were tested. Hypermethylation of the injected rDNA had no effect on transformation efficiency relative to mock-methylated controls. The injected C3 strain rDNA efficiently replaced host rDNA as the major constituent of the population of rDNA molecules. Hypermethylation of the injected DNA was not maintained through 20 to 25 cell generations.
author Karrer, K M
Yao, M C
author_facet Karrer, K M
Yao, M C
author_sort Karrer, K M
title Transformation of Tetrahymena thermophila with hypermethylated rRNA genes.
title_short Transformation of Tetrahymena thermophila with hypermethylated rRNA genes.
title_full Transformation of Tetrahymena thermophila with hypermethylated rRNA genes.
title_fullStr Transformation of Tetrahymena thermophila with hypermethylated rRNA genes.
title_full_unstemmed Transformation of Tetrahymena thermophila with hypermethylated rRNA genes.
title_sort transformation of tetrahymena thermophila with hypermethylated rrna genes.
publishDate 1988
url https://ncbi.nlm.nih.gov/pmc/articles/PMC363326/
https://ncbi.nlm.nih.gov/pubmed/3380092
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