Cell-type-dependent gene activation by yeast transposon Ty1 involves multiple regulatory determinants.

Ty transposable element insertion mutations of Saccharomyces cerevisiae can cause cell-type-dependent activation of adjacent gene expression. Several cis-acting regulatory regions within Ty1 that are responsible for these effects were identified. A 211-base-pair (bp) region functions as an activator...

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Main Authors: Company, M, Errede, B
Formato: Artigo
Idioma:en
Publicado em: 1987
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC367956/
https://ncbi.nlm.nih.gov/pubmed/2823122
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spelling pubmed-3679562004-03-19 Cell-type-dependent gene activation by yeast transposon Ty1 involves multiple regulatory determinants. Company, M Errede, B Mol Cell Biol Research Article Ty transposable element insertion mutations of Saccharomyces cerevisiae can cause cell-type-dependent activation of adjacent gene expression. Several cis-acting regulatory regions within Ty1 that are responsible for these effects were identified. A 211-base-pair (bp) region functions as an activator. This region includes the so-called U5 domain of delta and 145 bp of adjacent epsilon sequences. Unlike activation by the intact Ty1, activation by the 211-bp Ty1 subfragment is cell-type independent. The presence of a 112-bp fragment from a more distal region of Ty1 confers cell-type specificity to the activator. The 112-bp fragment includes sequences with homology to mammalian enhancers and to a yeast a/alpha control site. In addition, Ty1 regions that exert negative effects on gene expression were identified. These results demonstrate that the Ty1 transcriptional control region consists of multiple components with distinct regulatory functions. 1987-09 /pmc/articles/PMC367956/ /pubmed/2823122 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Company, M
Errede, B
Cell-type-dependent gene activation by yeast transposon Ty1 involves multiple regulatory determinants.
description Ty transposable element insertion mutations of Saccharomyces cerevisiae can cause cell-type-dependent activation of adjacent gene expression. Several cis-acting regulatory regions within Ty1 that are responsible for these effects were identified. A 211-base-pair (bp) region functions as an activator. This region includes the so-called U5 domain of delta and 145 bp of adjacent epsilon sequences. Unlike activation by the intact Ty1, activation by the 211-bp Ty1 subfragment is cell-type independent. The presence of a 112-bp fragment from a more distal region of Ty1 confers cell-type specificity to the activator. The 112-bp fragment includes sequences with homology to mammalian enhancers and to a yeast a/alpha control site. In addition, Ty1 regions that exert negative effects on gene expression were identified. These results demonstrate that the Ty1 transcriptional control region consists of multiple components with distinct regulatory functions.
author Company, M
Errede, B
author_facet Company, M
Errede, B
author_sort Company, M
title Cell-type-dependent gene activation by yeast transposon Ty1 involves multiple regulatory determinants.
title_short Cell-type-dependent gene activation by yeast transposon Ty1 involves multiple regulatory determinants.
title_full Cell-type-dependent gene activation by yeast transposon Ty1 involves multiple regulatory determinants.
title_fullStr Cell-type-dependent gene activation by yeast transposon Ty1 involves multiple regulatory determinants.
title_full_unstemmed Cell-type-dependent gene activation by yeast transposon Ty1 involves multiple regulatory determinants.
title_sort cell-type-dependent gene activation by yeast transposon ty1 involves multiple regulatory determinants.
publishDate 1987
url https://ncbi.nlm.nih.gov/pmc/articles/PMC367956/
https://ncbi.nlm.nih.gov/pubmed/2823122
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