Conditional Temperature-sensitive Restriction of Pseudomonas Bacteriophage CB3

Restriction of Pseudomonas bacteriophage CB3 growth on some Pseudomonas aeruginosa hosts was studied. On restricting hosts, growth of this phage was severely inhibited below 32 C and hence was temperature-sensitive. Investigation of this phenomenon revealed that restricting hosts were not killed as...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Olsen, R. H., Metcalf, Eleanor S., Brandt, Carl
Formato: Artigo
Idioma:en
Publicado em: 1968
Assuntos:
Bacterial Viruses
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC375484/
https://ncbi.nlm.nih.gov/pubmed/5745508
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
id pubmed-375484
record_format dspace
spelling pubmed-3754842004-05-13 Conditional Temperature-sensitive Restriction of Pseudomonas Bacteriophage CB3 Olsen, R. H. Metcalf, Eleanor S. Brandt, Carl J Virol Bacterial Viruses Restriction of Pseudomonas bacteriophage CB3 growth on some Pseudomonas aeruginosa hosts was studied. On restricting hosts, growth of this phage was severely inhibited below 32 C and hence was temperature-sensitive. Investigation of this phenomenon revealed that restricting hosts were not killed as a consequence of their infection under nonpermissive conditions. The ability of some hosts to restrict showed segregation in sexual crosses between restricting and nonrestricting hosts. However, the pattern of restriction among various hosts differed with the phage in question when other phages were compared with CB3. Temperature-shift experiments indicated that blockage of an early event in the phage lytic cycle occurred when restricting conditions were imposed on cells infected with CB3. This blockage could be eliminated by holding at permissive conditions until the cold-sensitive step was bypassed or by pulsing restricting cells for 5 min at 37 C. 1968-12 /pmc/articles/PMC375484/ /pubmed/5745508 Text en Copyright © 1968 American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Bacterial Viruses
spellingShingle Bacterial Viruses
Olsen, R. H.
Metcalf, Eleanor S.
Brandt, Carl
Conditional Temperature-sensitive Restriction of Pseudomonas Bacteriophage CB3
description Restriction of Pseudomonas bacteriophage CB3 growth on some Pseudomonas aeruginosa hosts was studied. On restricting hosts, growth of this phage was severely inhibited below 32 C and hence was temperature-sensitive. Investigation of this phenomenon revealed that restricting hosts were not killed as a consequence of their infection under nonpermissive conditions. The ability of some hosts to restrict showed segregation in sexual crosses between restricting and nonrestricting hosts. However, the pattern of restriction among various hosts differed with the phage in question when other phages were compared with CB3. Temperature-shift experiments indicated that blockage of an early event in the phage lytic cycle occurred when restricting conditions were imposed on cells infected with CB3. This blockage could be eliminated by holding at permissive conditions until the cold-sensitive step was bypassed or by pulsing restricting cells for 5 min at 37 C.
author Olsen, R. H.
Metcalf, Eleanor S.
Brandt, Carl
author_facet Olsen, R. H.
Metcalf, Eleanor S.
Brandt, Carl
author_sort Olsen, R. H.
title Conditional Temperature-sensitive Restriction of Pseudomonas Bacteriophage CB3
title_short Conditional Temperature-sensitive Restriction of Pseudomonas Bacteriophage CB3
title_full Conditional Temperature-sensitive Restriction of Pseudomonas Bacteriophage CB3
title_fullStr Conditional Temperature-sensitive Restriction of Pseudomonas Bacteriophage CB3
title_full_unstemmed Conditional Temperature-sensitive Restriction of Pseudomonas Bacteriophage CB3
title_sort conditional temperature-sensitive restriction of pseudomonas bacteriophage cb3
publishDate 1968
url https://ncbi.nlm.nih.gov/pmc/articles/PMC375484/
https://ncbi.nlm.nih.gov/pubmed/5745508
_version_ 1759802268266266624

Registos relacionados