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Indirect stimulation of genetic recombination
Recombination between lacZ alleles in crosses of λlacZ(1)(-) × λlacZ(2)(-) and F(-)lacZ(1)(-) × λlacZ(2)(-) in Escherichia coli (λ) can be stimulated manyfold by UV irradiation of one of the λlacZ phages [Porter, R. D., McLaughlin, T. & Low, B. (1979) Cold Spring Harbor Symp. Quant. Biol. 43, 10...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | en |
| Publicado em: |
1983
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC393605/ https://ncbi.nlm.nih.gov/pubmed/6219392 |
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| Resumo: | Recombination between lacZ alleles in crosses of λlacZ(1)(-) × λlacZ(2)(-) and F(-)lacZ(1)(-) × λlacZ(2)(-) in Escherichia coli (λ) can be stimulated manyfold by UV irradiation of one of the λlacZ phages [Porter, R. D., McLaughlin, T. & Low, B. (1979) Cold Spring Harbor Symp. Quant. Biol. 43, 1043-1048]. Analogous stimulation has now been observed by coinfection of the cells by UV-irradiated λ phage which carries no lac region. This indirect stimulation is not dependent on induction of the SOS system. The bacterial uvr system can effectively remove the damages on the λ DNA which cause the indirect stimulation. Among a number of mutations tested, only ssb-1 was found to cause a drastic decrease in the indirect stimulation. Indirect stimulation was caused only by using phage that had a region of homology with the recombining phage. The homologous region can be separated from the recombining region by an extended nonhomologous region (>7.9 × 10(3) base pairs). This implies that damages to the DNA molecule, which stimulate recombination, can be located very far from the recombining region of the molecule. |
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