Kinetics of cytokine expression during primary human immunodeficiency virus type 1 infection.

In the present study, we have determined the kinetics of constitutive expression of a panel of cytokines [interleukin (IL) 2, IL-4, IL-6, IL-10, interferon gamma (IFN-gamma), and tumor necrosis factor alpha (TNF-alpha)] in sequential peripheral blood mononuclear cell samples from nine individuals wi...

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Autors principals: Graziosi, C, Gantt, K R, Vaccarezza, M, Demarest, J F, Daucher, M, Saag, M S, Shaw, G M, Quinn, T C, Cohen, O J, Welbon, C C, Pantaleo, G, Fauci, A S
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Publicat: 1996
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Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC39547/
https://ncbi.nlm.nih.gov/pubmed/8633076
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spelling pubmed-395472001-08-25 Kinetics of cytokine expression during primary human immunodeficiency virus type 1 infection. Graziosi, C Gantt, K R Vaccarezza, M Demarest, J F Daucher, M Saag, M S Shaw, G M Quinn, T C Cohen, O J Welbon, C C Pantaleo, G Fauci, A S Proc Natl Acad Sci U S A Research Article In the present study, we have determined the kinetics of constitutive expression of a panel of cytokines [interleukin (IL) 2, IL-4, IL-6, IL-10, interferon gamma (IFN-gamma), and tumor necrosis factor alpha (TNF-alpha)] in sequential peripheral blood mononuclear cell samples from nine individuals with primary human immunodeficiency virus infection. Expression of IL-2 and IL-4 was barely detected in peripheral blood mononuclear cells. However, substantial levels of IL-2 expression were found in mononuclear cells isolated from lymph node. Expression of IL-6 was detected in only three of nine patients, and IL-6 expression was observed when transition from the acute to the chronic phase had already occurred. Expression of IL-10 and TNF-alpha was consistently observed in all patients tested, and levels of both cytokines were either stable or progressively increased over time. Similar to IL-10 and TNF-alpha, IFN-gamma expression was detected in all patients; however, in five of nine patients, IFN-gamma expression peaked very early during primary infection. The early peak in IFN-gamma expression coincided with oligoclonal expansions of CD8+ T cells in five of six patients, and CD8+ T cells mostly accounted for the expression of this cytokine. These results indicate that high levels of expression of proinflammatory cytokines are associated with primary infection and that the cytokine response during this phase of infection is strongly influenced by oligoclonal expansions of CD8+ T cells. 1996-04-30 /pmc/articles/PMC39547/ /pubmed/8633076 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Graziosi, C
Gantt, K R
Vaccarezza, M
Demarest, J F
Daucher, M
Saag, M S
Shaw, G M
Quinn, T C
Cohen, O J
Welbon, C C
Pantaleo, G
Fauci, A S
Kinetics of cytokine expression during primary human immunodeficiency virus type 1 infection.
description In the present study, we have determined the kinetics of constitutive expression of a panel of cytokines [interleukin (IL) 2, IL-4, IL-6, IL-10, interferon gamma (IFN-gamma), and tumor necrosis factor alpha (TNF-alpha)] in sequential peripheral blood mononuclear cell samples from nine individuals with primary human immunodeficiency virus infection. Expression of IL-2 and IL-4 was barely detected in peripheral blood mononuclear cells. However, substantial levels of IL-2 expression were found in mononuclear cells isolated from lymph node. Expression of IL-6 was detected in only three of nine patients, and IL-6 expression was observed when transition from the acute to the chronic phase had already occurred. Expression of IL-10 and TNF-alpha was consistently observed in all patients tested, and levels of both cytokines were either stable or progressively increased over time. Similar to IL-10 and TNF-alpha, IFN-gamma expression was detected in all patients; however, in five of nine patients, IFN-gamma expression peaked very early during primary infection. The early peak in IFN-gamma expression coincided with oligoclonal expansions of CD8+ T cells in five of six patients, and CD8+ T cells mostly accounted for the expression of this cytokine. These results indicate that high levels of expression of proinflammatory cytokines are associated with primary infection and that the cytokine response during this phase of infection is strongly influenced by oligoclonal expansions of CD8+ T cells.
author Graziosi, C
Gantt, K R
Vaccarezza, M
Demarest, J F
Daucher, M
Saag, M S
Shaw, G M
Quinn, T C
Cohen, O J
Welbon, C C
Pantaleo, G
Fauci, A S
author_facet Graziosi, C
Gantt, K R
Vaccarezza, M
Demarest, J F
Daucher, M
Saag, M S
Shaw, G M
Quinn, T C
Cohen, O J
Welbon, C C
Pantaleo, G
Fauci, A S
author_sort Graziosi, C
title Kinetics of cytokine expression during primary human immunodeficiency virus type 1 infection.
title_short Kinetics of cytokine expression during primary human immunodeficiency virus type 1 infection.
title_full Kinetics of cytokine expression during primary human immunodeficiency virus type 1 infection.
title_fullStr Kinetics of cytokine expression during primary human immunodeficiency virus type 1 infection.
title_full_unstemmed Kinetics of cytokine expression during primary human immunodeficiency virus type 1 infection.
title_sort kinetics of cytokine expression during primary human immunodeficiency virus type 1 infection.
publishDate 1996
url https://ncbi.nlm.nih.gov/pmc/articles/PMC39547/
https://ncbi.nlm.nih.gov/pubmed/8633076
_version_ 1758994809330073600

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