Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.

An oligonucleotide hybridization procedure has been developed that eliminates the preferential melting of A X T versus G X C base pairs, allowing the stringency of the hybridization to be controlled as a function of probe length only. This technique, which uses tetramethylammonium chloride, is espec...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Wood, W I, Gitschier, J, Lasky, L A, Lawn, R M
Formato: Artigo
Idioma:en
Publicado em: 1985
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC397316/
https://ncbi.nlm.nih.gov/pubmed/3856838
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
id pubmed-397316
record_format dspace
spelling pubmed-3973162004-04-27 Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries. Wood, W I Gitschier, J Lasky, L A Lawn, R M Proc Natl Acad Sci U S A Research Article An oligonucleotide hybridization procedure has been developed that eliminates the preferential melting of A X T versus G X C base pairs, allowing the stringency of the hybridization to be controlled as a function of probe length only. This technique, which uses tetramethylammonium chloride, is especially helpful whenever a highly complex library is screened with a pool of oligonucleotide probes, which usually vary widely in base composition. The procedure can also be applied advantageously whenever an exact match to an oligonucleotide probe is desired, such as in screening for clones having as little as a single-base alteration generated by in vitro mutagenesis. 1985-03 /pmc/articles/PMC397316/ /pubmed/3856838 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Wood, W I
Gitschier, J
Lasky, L A
Lawn, R M
Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.
description An oligonucleotide hybridization procedure has been developed that eliminates the preferential melting of A X T versus G X C base pairs, allowing the stringency of the hybridization to be controlled as a function of probe length only. This technique, which uses tetramethylammonium chloride, is especially helpful whenever a highly complex library is screened with a pool of oligonucleotide probes, which usually vary widely in base composition. The procedure can also be applied advantageously whenever an exact match to an oligonucleotide probe is desired, such as in screening for clones having as little as a single-base alteration generated by in vitro mutagenesis.
author Wood, W I
Gitschier, J
Lasky, L A
Lawn, R M
author_facet Wood, W I
Gitschier, J
Lasky, L A
Lawn, R M
author_sort Wood, W I
title Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.
title_short Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.
title_full Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.
title_fullStr Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.
title_full_unstemmed Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.
title_sort base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.
publishDate 1985
url https://ncbi.nlm.nih.gov/pmc/articles/PMC397316/
https://ncbi.nlm.nih.gov/pubmed/3856838
_version_ 1759804903340900352

Registos relacionados