Characterization of enterotoxin purified from Clostridium perfringens type C.

Enterotoxin produced by a sporulating culture of Clostridium perfringens type C, which had been isolated from a case of severe necrotic enteritis, was purified. The molecular weight was estimated to be 36,000 by gel chromatography on Sephadex G-100 and 33,400 by ultracentrifugation. The sedimentatio...

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Main Authors: Skjelkvålé, R, Duncan, C L
Formato: Artigo
Idioma:en
Publicado em: 1975
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC415180/
https://ncbi.nlm.nih.gov/pubmed/164411
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spelling pubmed-4151802004-07-07 Characterization of enterotoxin purified from Clostridium perfringens type C. Skjelkvålé, R Duncan, C L Infect Immun Research Article Enterotoxin produced by a sporulating culture of Clostridium perfringens type C, which had been isolated from a case of severe necrotic enteritis, was purified. The molecular weight was estimated to be 36,000 by gel chromatography on Sephadex G-100 and 33,400 by ultracentrifugation. The sedimentation coefficient S20,W was 2.92. The toxin protein exhibited unusual behavior on sodium dodecyl sulfate gels, and toxin aggregates having molecular weights of 68,000, 85,000, 105,000, and 140,000 were obtained. The purified enterotoxin often separated, apparently due to slight charge differences, into two protein bands on 7% polyacrylamide gels. Electrofocusing of enterotoxin on polyacrylamide gels gave an approximate isoelectric point of 4.3, with the enterotoxin being fractionated into four distinct protein bands. The specific toxicity of the enterotoxin was about 1,900 mouse mean lethal doses per mg of calculated nitrogen. The data obtained indicate that the enterotoxin from C. perfringens type C is identical in most respects to that obtained from type A strains. Whether or not this toxin plays a role in the necrotic enteritis caused by type C strains is unknown at present. 1975-05 /pmc/articles/PMC415180/ /pubmed/164411 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Skjelkvålé, R
Duncan, C L
Characterization of enterotoxin purified from Clostridium perfringens type C.
description Enterotoxin produced by a sporulating culture of Clostridium perfringens type C, which had been isolated from a case of severe necrotic enteritis, was purified. The molecular weight was estimated to be 36,000 by gel chromatography on Sephadex G-100 and 33,400 by ultracentrifugation. The sedimentation coefficient S20,W was 2.92. The toxin protein exhibited unusual behavior on sodium dodecyl sulfate gels, and toxin aggregates having molecular weights of 68,000, 85,000, 105,000, and 140,000 were obtained. The purified enterotoxin often separated, apparently due to slight charge differences, into two protein bands on 7% polyacrylamide gels. Electrofocusing of enterotoxin on polyacrylamide gels gave an approximate isoelectric point of 4.3, with the enterotoxin being fractionated into four distinct protein bands. The specific toxicity of the enterotoxin was about 1,900 mouse mean lethal doses per mg of calculated nitrogen. The data obtained indicate that the enterotoxin from C. perfringens type C is identical in most respects to that obtained from type A strains. Whether or not this toxin plays a role in the necrotic enteritis caused by type C strains is unknown at present.
author Skjelkvålé, R
Duncan, C L
author_facet Skjelkvålé, R
Duncan, C L
author_sort Skjelkvålé, R
title Characterization of enterotoxin purified from Clostridium perfringens type C.
title_short Characterization of enterotoxin purified from Clostridium perfringens type C.
title_full Characterization of enterotoxin purified from Clostridium perfringens type C.
title_fullStr Characterization of enterotoxin purified from Clostridium perfringens type C.
title_full_unstemmed Characterization of enterotoxin purified from Clostridium perfringens type C.
title_sort characterization of enterotoxin purified from clostridium perfringens type c.
publishDate 1975
url https://ncbi.nlm.nih.gov/pmc/articles/PMC415180/
https://ncbi.nlm.nih.gov/pubmed/164411
_version_ 1759806273622114304

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