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A GFP-based assay for rapid screening of compounds affecting ARE-dependent mRNA turnover
A reporter transcript containing the green fluorescent protein (GFP) gene upstream of the destabilizing 3′-untranslated region (3′-UTR) of the murine IL-3 gene was inserted in mouse PB-3c-15 mast cells. The GFP–IL-3 transcript was inherently unstable due to the presence of an adenosine–uridine (AU)-...
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| Hlavní autoři: | , , |
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| Médium: | Článek |
| Jazyk: | en |
| Vydáno: |
Oxford University Press
2004
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC443554/ https://ncbi.nlm.nih.gov/pubmed/15247322 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh086 |
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