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Fluorescence lifetime imaging of free and protein-bound NADH.
We introduce a methodology, fluorescence lifetime imaging (FLIM), in which the contrast depends on the fluorescence lifetime at each point in a two-dimensional image and not on the local concentration and/or intensity of the fluorophore. We used FLIM to create lifetime images of NADH when free in so...
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| Autori principali: | , , , |
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| Natura: | Articolo |
| Lingua: | en |
| Pubblicazione: |
1992
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC48431/ https://ncbi.nlm.nih.gov/pubmed/1741380 |
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