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Real-Time TaqMan PCR for Quantifying Oral Bacteria during Biofilm Formation

A TaqMan PCR was developed for quantifying early colonizer microorganisms in dental biofilms. To design species-specific primers and TaqMan probes, genomic subtractive hybridization was used. This quantitative assay in combination with subtractive hybridization may be of value in the study of microb...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Suzuki, Nao, Nakano, Yoshio, Yoshida, Akihiro, Yamashita, Yoshihisa, Kiyoura, Yusuke
Format: Artikel
Sprache:English
Veröffentlicht: American Society for Microbiology 2004
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC497611/
https://ncbi.nlm.nih.gov/pubmed/15297540
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.8.3827-3830.2004
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