CA 125 secretion by peritoneal mesothelial cells.

AIMS--To investigate the secretion of the tumour marker CA 125 by cultured human mesothelial cells; to determine if secretion of CA 125 could be observed by activating the mesothelial monolayers with different cytokines. METHODS--Mesothelial cells were isolated from human omentum and cultured to con...

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Main Authors: Zeillemaker, A M, Verbrugh, H A, Hoynck van Papendrecht, A A, Leguit, P
Formato: Artigo
Idioma:English
Publicado: 1994
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC501908/
https://ncbi.nlm.nih.gov/pubmed/8163699
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spelling pubmed-5019082005-10-03 CA 125 secretion by peritoneal mesothelial cells. Zeillemaker, A M Verbrugh, H A Hoynck van Papendrecht, A A Leguit, P J Clin Pathol Research Article AIMS--To investigate the secretion of the tumour marker CA 125 by cultured human mesothelial cells; to determine if secretion of CA 125 could be observed by activating the mesothelial monolayers with different cytokines. METHODS--Mesothelial cells were isolated from human omentum and cultured to confluent monolayers on perforated polycarbonate membranes (pore size 0.4 micron). The mesothelial monolayers were activated and the apical and basolateral secretion of CA 125 compared. To investigate the influence of cytokines, mesothelial cells were cultured and activated with recombinant interleukin-1 beta (rIL-1 beta), tumour necrosis factor-alpha (TNF-alpha) or lipopolysaccharide from Escherichia coli. The secretion of CA 125 was tested using a microparticle enzyme immunoassay. RESULTS--Mesothelial monolayers secreted CA 125 in a polarised manner with preference for the apical side. Apical polarisation occurred irrespective of the side of the inducing stimulus (p < or = 0.05). Non-activated cultured mesothelial monolayers secreted significant quantities of CA 125, indicating constitutive production of this protein. However, CA 125 production was significantly enhanced if mesothelial cells were incubated with rIL-1 beta (p < or = 0.05), TNF-alpha (p < or = 0.05), and E coli LPS (p < or = 0.01). CONCLUSIONS--Human mesothelial monolayers secrete CA 125 preferentially from their apical surfaces. The secretion of CA 125 can be enhanced by the inflammatory cytokines Il-1 beta, TNF-alpha, and by E coli LPS. 1994-03 /pmc/articles/PMC501908/ /pubmed/8163699 Text en
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Research Article
spellingShingle Research Article
Zeillemaker, A M
Verbrugh, H A
Hoynck van Papendrecht, A A
Leguit, P
CA 125 secretion by peritoneal mesothelial cells.
description AIMS--To investigate the secretion of the tumour marker CA 125 by cultured human mesothelial cells; to determine if secretion of CA 125 could be observed by activating the mesothelial monolayers with different cytokines. METHODS--Mesothelial cells were isolated from human omentum and cultured to confluent monolayers on perforated polycarbonate membranes (pore size 0.4 micron). The mesothelial monolayers were activated and the apical and basolateral secretion of CA 125 compared. To investigate the influence of cytokines, mesothelial cells were cultured and activated with recombinant interleukin-1 beta (rIL-1 beta), tumour necrosis factor-alpha (TNF-alpha) or lipopolysaccharide from Escherichia coli. The secretion of CA 125 was tested using a microparticle enzyme immunoassay. RESULTS--Mesothelial monolayers secreted CA 125 in a polarised manner with preference for the apical side. Apical polarisation occurred irrespective of the side of the inducing stimulus (p < or = 0.05). Non-activated cultured mesothelial monolayers secreted significant quantities of CA 125, indicating constitutive production of this protein. However, CA 125 production was significantly enhanced if mesothelial cells were incubated with rIL-1 beta (p < or = 0.05), TNF-alpha (p < or = 0.05), and E coli LPS (p < or = 0.01). CONCLUSIONS--Human mesothelial monolayers secrete CA 125 preferentially from their apical surfaces. The secretion of CA 125 can be enhanced by the inflammatory cytokines Il-1 beta, TNF-alpha, and by E coli LPS.
author Zeillemaker, A M
Verbrugh, H A
Hoynck van Papendrecht, A A
Leguit, P
author_facet Zeillemaker, A M
Verbrugh, H A
Hoynck van Papendrecht, A A
Leguit, P
author_sort Zeillemaker, A M
title CA 125 secretion by peritoneal mesothelial cells.
title_short CA 125 secretion by peritoneal mesothelial cells.
title_full CA 125 secretion by peritoneal mesothelial cells.
title_fullStr CA 125 secretion by peritoneal mesothelial cells.
title_full_unstemmed CA 125 secretion by peritoneal mesothelial cells.
title_sort ca 125 secretion by peritoneal mesothelial cells.
publishDate 1994
url https://ncbi.nlm.nih.gov/pmc/articles/PMC501908/
https://ncbi.nlm.nih.gov/pubmed/8163699
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