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Allelic diversity of S-RNase alleles in diploid potato species
KEY MESSAGE: The S-ribonuclease sequences of 16 S-alleles derived from diploid types of Solanum are presented. A phylogenetic analysis and partial phenotypic analysis support the conclusion that these are functional S-alleles. ABSTRACT: S-Ribonucleases (S-RNases) control the pistil specificity of th...
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| Format: | Article |
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| Idioma: | en |
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Springer Berlin Heidelberg
2016
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| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5025496/ https://ncbi.nlm.nih.gov/pubmed/27497984 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00122-016-2754-7 |
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pubmed-5025496 |
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| record_format |
dspace |
| institution |
US National Library of Medicine |
| collection |
PubMed Central |
| language |
en |
| format |
Article |
| topic |
Original Article |
| spellingShingle |
Original Article Allelic diversity of S-RNase alleles in diploid potato species |
| topic_facet |
Original Article |
| description |
KEY MESSAGE: The S-ribonuclease sequences of 16 S-alleles derived from diploid types of Solanum are presented. A phylogenetic analysis and partial phenotypic analysis support the conclusion that these are functional S-alleles. ABSTRACT: S-Ribonucleases (S-RNases) control the pistil specificity of the self-incompatibility (SI) response in the genus Solanum and several other members of the Solanaceae. The nucleotide sequences of S-RNases corresponding to a large number of S-alleles or S-haplotypes have been characterised. However, surprisingly, few S-RNase sequences are available for potato species. The identification of new S-alleles in diploid potato species is desirable as these stocks are important sources of traits such as biotic and abiotic resistance. S-RNase sequences are reported here from three distinct diploid types of potato: cultivated Solanum tuberosum Group Phureja, S. tuberosum Group Stenotomum, and the wild species Solanum okadae. Partial S-RNase sequences were obtained from pistil RNA by RT-PCR or 3′RACE (Rapid Amplification of cDNA Ends) using a degenerate primer. Full-length sequences were obtained for two alleles by 5′RACE. Database searches with these sequences identified 16 S-RNases in total, all of which are novel. The sequence analysis revealed all the expected features of functional S-RNases. Phylogenetic analysis with selected published S-RNase and S-like-RNase sequences from the Solanaceae revealed extensive trans-generic evolution of the S-RNases and a clear distinction from S-like-RNases. Pollination tests were used to confirm the self-incompatibility status and cross-compatibility relationships of the S. okadae accessions. All the S. okadae accessions were found to be self-incompatible as expected with crosses amongst them exhibiting both cross-compatibility and semi-compatibility consistent with the S-genotypes determined from the S-RNase sequence data. The progeny analysis of four semi-compatible crosses examined by allele-specific PCR provided further confirmation that these are functional S-RNases. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00122-016-2754-7) contains supplementary material, which is available to authorized users. |
| author_sort |
Dzidzienyo, Daniel K. |
| title |
Allelic diversity of S-RNase alleles in diploid potato species |
| title_short |
Allelic diversity of S-RNase alleles in diploid potato species |
| title_full |
Allelic diversity of S-RNase alleles in diploid potato species |
| title_fullStr |
Allelic diversity of S-RNase alleles in diploid potato species |
| title_full_unstemmed |
Allelic diversity of S-RNase alleles in diploid potato species |
| title_sort |
allelic diversity of s-rnase alleles in diploid potato species |
| publisher |
Springer Berlin Heidelberg |
| publisher_facet |
Springer Berlin Heidelberg |
| publishDate |
2016 |
| url |
https://ncbi.nlm.nih.gov/pmc/articles/PMC5025496/ https://ncbi.nlm.nih.gov/pubmed/27497984 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00122-016-2754-7 |
| _version_ |
1872506675055820801 |