Collagen types I and III in giant papillae of vernal keratoconjunctivitis.

AIMS--The objective of this study was to investigate alterations in conjunctival collagen and proteoglycans in the conjunctival giant papillae of patients with vernal keratoconjunctivitis (VKC). METHODS--Tissue samples from tarsal giant papillae of seven eyes from five patients with VKC, and five ta...

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Main Authors: Leonardi, A, Abatangelo, G, Cortivo, R, Secchi, A G
Formato: Artigo
Idioma:English
Publicado em: 1995
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC505140/
https://ncbi.nlm.nih.gov/pubmed/7612563
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spelling pubmed-5051402007-07-03 Collagen types I and III in giant papillae of vernal keratoconjunctivitis. Leonardi, A Abatangelo, G Cortivo, R Secchi, A G Br J Ophthalmol Research Article AIMS--The objective of this study was to investigate alterations in conjunctival collagen and proteoglycans in the conjunctival giant papillae of patients with vernal keratoconjunctivitis (VKC). METHODS--Tissue samples from tarsal giant papillae of seven eyes from five patients with VKC, and five tarsal conjunctival samples from five normal patients were obtained. Tissues were processed and stained with haematoxylin and eosin, Van Gieson, trichromic Mallory, toluidine blue, Alcian blue, and alkaline Giemsa. Collagen extraction was performed in acetic acid and pepsin, total collagen was quantified using hydroxy-proline levels, and collagen types I and III were analysed by gel electrophoresis (SDS-PAGE). Proteoglycans were quantified using uronic acid levels. RESULTS--Histological evaluation showed a significant increase of mast cells in the epithelium (0/mm2 v 147/mm2, p < 0.01) and in the stroma (5.1/mm2 v 80/mm2, p < 0.01) of VKC patients. Collagen fibres were thicker and arranged irregularly, with the total amount significantly increased. Owing to an increased percentage of type III collagen, the ratio of collagen types I to III was decreased. Proteoglycans were also reduced in VKC samples. CONCLUSION--The well known morphological abnormalities observed in VKC correspond to alterations in the ratio between collagens and proteoglycans, and between different types of collagen. The greatly increased number of mast cells found in these tissues suggests an active role for these cells in the abnormal connective tissue metabolism observed in VKC. 1995-05 /pmc/articles/PMC505140/ /pubmed/7612563 Text en
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Research Article
spellingShingle Research Article
Leonardi, A
Abatangelo, G
Cortivo, R
Secchi, A G
Collagen types I and III in giant papillae of vernal keratoconjunctivitis.
description AIMS--The objective of this study was to investigate alterations in conjunctival collagen and proteoglycans in the conjunctival giant papillae of patients with vernal keratoconjunctivitis (VKC). METHODS--Tissue samples from tarsal giant papillae of seven eyes from five patients with VKC, and five tarsal conjunctival samples from five normal patients were obtained. Tissues were processed and stained with haematoxylin and eosin, Van Gieson, trichromic Mallory, toluidine blue, Alcian blue, and alkaline Giemsa. Collagen extraction was performed in acetic acid and pepsin, total collagen was quantified using hydroxy-proline levels, and collagen types I and III were analysed by gel electrophoresis (SDS-PAGE). Proteoglycans were quantified using uronic acid levels. RESULTS--Histological evaluation showed a significant increase of mast cells in the epithelium (0/mm2 v 147/mm2, p < 0.01) and in the stroma (5.1/mm2 v 80/mm2, p < 0.01) of VKC patients. Collagen fibres were thicker and arranged irregularly, with the total amount significantly increased. Owing to an increased percentage of type III collagen, the ratio of collagen types I to III was decreased. Proteoglycans were also reduced in VKC samples. CONCLUSION--The well known morphological abnormalities observed in VKC correspond to alterations in the ratio between collagens and proteoglycans, and between different types of collagen. The greatly increased number of mast cells found in these tissues suggests an active role for these cells in the abnormal connective tissue metabolism observed in VKC.
author Leonardi, A
Abatangelo, G
Cortivo, R
Secchi, A G
author_facet Leonardi, A
Abatangelo, G
Cortivo, R
Secchi, A G
author_sort Leonardi, A
title Collagen types I and III in giant papillae of vernal keratoconjunctivitis.
title_short Collagen types I and III in giant papillae of vernal keratoconjunctivitis.
title_full Collagen types I and III in giant papillae of vernal keratoconjunctivitis.
title_fullStr Collagen types I and III in giant papillae of vernal keratoconjunctivitis.
title_full_unstemmed Collagen types I and III in giant papillae of vernal keratoconjunctivitis.
title_sort collagen types i and iii in giant papillae of vernal keratoconjunctivitis.
publishDate 1995
url https://ncbi.nlm.nih.gov/pmc/articles/PMC505140/
https://ncbi.nlm.nih.gov/pubmed/7612563
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