Cloning and expression of a cDNA encoding the transporter of taurine and beta-alanine in mouse brain.

A taurine/beta-alanine transporter was cloned from a mouse brain cDNA library by screening with a partial cDNA probe of the glycine transporter at low stringency. The deduced amino acid sequence predicts 590 amino acids with typical characteristics of the sodium-dependent neurotransmitter transporte...

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Autors principals: Liu, Q R, López-Corcuera, B, Nelson, H, Mandiyan, S, Nelson, N
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Idioma:en
Publicat: 1992
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Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC50715/
https://ncbi.nlm.nih.gov/pubmed/1465453
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spelling pubmed-507152001-08-25 Cloning and expression of a cDNA encoding the transporter of taurine and beta-alanine in mouse brain. Liu, Q R López-Corcuera, B Nelson, H Mandiyan, S Nelson, N Proc Natl Acad Sci U S A Research Article A taurine/beta-alanine transporter was cloned from a mouse brain cDNA library by screening with a partial cDNA probe of the glycine transporter at low stringency. The deduced amino acid sequence predicts 590 amino acids with typical characteristics of the sodium-dependent neurotransmitter transporters such as sequence homology and membrane topography. However, the calculated isoelectric point of the taurine/beta-alanine transporter is more acidic (pI = 5.98) than those (pI > 8.0) of other cloned neurotransmitter transporters. Xenopus oocytes injected with cRNA of the cloned transporter expressed uptake activities with Km = 4.5 microM for taurine and Km = 56 microM for beta-alanine. Northern hybridization showed a single transcript of 7.5 kilobases that was highly enriched in kidney and distributed evenly in various parts of the brain. In situ hybridization showed the mRNA of the taurine/beta-alanine transporter to be localized in the corpus callosum, striatum, and anterior commisure. Specific localization of the taurine/beta-alanine transporter in mouse brain suggests a potential function for taurine and beta-alanine as neurotransmitters. 1992-12-15 /pmc/articles/PMC50715/ /pubmed/1465453 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Liu, Q R
López-Corcuera, B
Nelson, H
Mandiyan, S
Nelson, N
Cloning and expression of a cDNA encoding the transporter of taurine and beta-alanine in mouse brain.
description A taurine/beta-alanine transporter was cloned from a mouse brain cDNA library by screening with a partial cDNA probe of the glycine transporter at low stringency. The deduced amino acid sequence predicts 590 amino acids with typical characteristics of the sodium-dependent neurotransmitter transporters such as sequence homology and membrane topography. However, the calculated isoelectric point of the taurine/beta-alanine transporter is more acidic (pI = 5.98) than those (pI > 8.0) of other cloned neurotransmitter transporters. Xenopus oocytes injected with cRNA of the cloned transporter expressed uptake activities with Km = 4.5 microM for taurine and Km = 56 microM for beta-alanine. Northern hybridization showed a single transcript of 7.5 kilobases that was highly enriched in kidney and distributed evenly in various parts of the brain. In situ hybridization showed the mRNA of the taurine/beta-alanine transporter to be localized in the corpus callosum, striatum, and anterior commisure. Specific localization of the taurine/beta-alanine transporter in mouse brain suggests a potential function for taurine and beta-alanine as neurotransmitters.
author Liu, Q R
López-Corcuera, B
Nelson, H
Mandiyan, S
Nelson, N
author_facet Liu, Q R
López-Corcuera, B
Nelson, H
Mandiyan, S
Nelson, N
author_sort Liu, Q R
title Cloning and expression of a cDNA encoding the transporter of taurine and beta-alanine in mouse brain.
title_short Cloning and expression of a cDNA encoding the transporter of taurine and beta-alanine in mouse brain.
title_full Cloning and expression of a cDNA encoding the transporter of taurine and beta-alanine in mouse brain.
title_fullStr Cloning and expression of a cDNA encoding the transporter of taurine and beta-alanine in mouse brain.
title_full_unstemmed Cloning and expression of a cDNA encoding the transporter of taurine and beta-alanine in mouse brain.
title_sort cloning and expression of a cdna encoding the transporter of taurine and beta-alanine in mouse brain.
publishDate 1992
url https://ncbi.nlm.nih.gov/pmc/articles/PMC50715/
https://ncbi.nlm.nih.gov/pubmed/1465453
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