An efficient one-step site-directed and site-saturation mutagenesis protocol

We have developed a new primer design method based on the QuickChange™ site-directed mutagenesis protocol, which significantly improves the PCR amplification efficiency. This design method minimizes primer dimerization and ensures the priority of primer-template annealing over primer self-pairing du...

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Bibliografische gegevens
Hoofdauteurs: Zheng, Lei, Baumann, Ulrich, Reymond, Jean-Louis
Formaat: Artikel
Taal:English
Gepubliceerd in: Oxford University Press 2004
Onderwerpen:
NAR Methods Online
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC514394/
https://ncbi.nlm.nih.gov/pubmed/15304544
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh110
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Internet

https://ncbi.nlm.nih.gov/pmc/articles/PMC514394/
https://ncbi.nlm.nih.gov/pubmed/15304544
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh110

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