Interaction of Simian Virus 40 chromatin with Simian Virus 40 T-antigen.

We have studied the binding of the tumor antigen (T-antigen) of simian virus 40 to simian virus 40 chromatin (minichromosomes). The minichromosomes isolated from infected cells by a modification of standard techniques were relatively free of contaminating RNA and cellular DNA and had a ratio (by wei...

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Main Authors: Persico-DiLauro, M, Martin, R G, Livingston, D M
Formato: Artigo
Idioma:English
Publicado em: 1977
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC515954/
https://ncbi.nlm.nih.gov/pubmed/199733
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spelling pubmed-5159542004-09-08 Interaction of Simian Virus 40 chromatin with Simian Virus 40 T-antigen. Persico-DiLauro, M Martin, R G Livingston, D M J Virol Research Article We have studied the binding of the tumor antigen (T-antigen) of simian virus 40 to simian virus 40 chromatin (minichromosomes). The minichromosomes isolated from infected cells by a modification of standard techniques were relatively free of contaminating RNA and cellular DNA and had a ratio (by weight) of protein to DNA of approximately 1; their DNA was 50 to 60% digestible to an acid-soluble form by staphylococcal nuclease. Cleavage of this chromatin with restriction endonucleases indicated that the nuclease-resistant regions were randomly distributed in the population of minichromosomes, but were not randomly distributed within minichromosomes. Only 20 to 35% of these minichromosomes adsorbed nonspecifically to nitrocellulose filters, permitting binding studies between simian virus 40 T-antigen and chromatin to be performed. Approximately two to three times as much T-antigen was required to bind chromatin as to bind an equivalent amount of free DNA. When T-antigen was present in excess, both chromatin and free DNA were quantitatively retained on the filters. On the other hand, when DNA or chromatin was present in excess, only one-third as much chromatin as DNA was retained. We suggest that T-antigen-chromatin complexes may be formed by the cooperative binding of T-antigen to chromatin, whereas T-antigen-DNA complexes may be formed by simple bimolecular interactions. 1977-11 /pmc/articles/PMC515954/ /pubmed/199733 Text en
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Research Article
spellingShingle Research Article
Persico-DiLauro, M
Martin, R G
Livingston, D M
Interaction of Simian Virus 40 chromatin with Simian Virus 40 T-antigen.
description We have studied the binding of the tumor antigen (T-antigen) of simian virus 40 to simian virus 40 chromatin (minichromosomes). The minichromosomes isolated from infected cells by a modification of standard techniques were relatively free of contaminating RNA and cellular DNA and had a ratio (by weight) of protein to DNA of approximately 1; their DNA was 50 to 60% digestible to an acid-soluble form by staphylococcal nuclease. Cleavage of this chromatin with restriction endonucleases indicated that the nuclease-resistant regions were randomly distributed in the population of minichromosomes, but were not randomly distributed within minichromosomes. Only 20 to 35% of these minichromosomes adsorbed nonspecifically to nitrocellulose filters, permitting binding studies between simian virus 40 T-antigen and chromatin to be performed. Approximately two to three times as much T-antigen was required to bind chromatin as to bind an equivalent amount of free DNA. When T-antigen was present in excess, both chromatin and free DNA were quantitatively retained on the filters. On the other hand, when DNA or chromatin was present in excess, only one-third as much chromatin as DNA was retained. We suggest that T-antigen-chromatin complexes may be formed by the cooperative binding of T-antigen to chromatin, whereas T-antigen-DNA complexes may be formed by simple bimolecular interactions.
author Persico-DiLauro, M
Martin, R G
Livingston, D M
author_facet Persico-DiLauro, M
Martin, R G
Livingston, D M
author_sort Persico-DiLauro, M
title Interaction of Simian Virus 40 chromatin with Simian Virus 40 T-antigen.
title_short Interaction of Simian Virus 40 chromatin with Simian Virus 40 T-antigen.
title_full Interaction of Simian Virus 40 chromatin with Simian Virus 40 T-antigen.
title_fullStr Interaction of Simian Virus 40 chromatin with Simian Virus 40 T-antigen.
title_full_unstemmed Interaction of Simian Virus 40 chromatin with Simian Virus 40 T-antigen.
title_sort interaction of simian virus 40 chromatin with simian virus 40 t-antigen.
publishDate 1977
url https://ncbi.nlm.nih.gov/pmc/articles/PMC515954/
https://ncbi.nlm.nih.gov/pubmed/199733
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