Localization of stromelysin gene expression in atherosclerotic plaques by in situ hybridization.

The stromelysins are members of a family of extracellular matrix metalloproteinases. These enzymes may erode the connective tissue in atherosclerotic plaques, leading to fissuring and acute thrombotic events. Cell-specific stromelysin expression in human atherosclerotic plaques was studied by in sit...

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Những tác giả chính: Henney, A M, Wakeley, P R, Davies, M J, Foster, K, Hembry, R, Murphy, G, Humphries, S
Định dạng: Bài viết
Ngôn ngữ:en
Được phát hành: 1991
Những chủ đề:
Research Article
Truy cập trực tuyến:https://ncbi.nlm.nih.gov/pmc/articles/PMC52465/
https://ncbi.nlm.nih.gov/pubmed/1896464
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spelling pubmed-524652001-08-25 Localization of stromelysin gene expression in atherosclerotic plaques by in situ hybridization. Henney, A M Wakeley, P R Davies, M J Foster, K Hembry, R Murphy, G Humphries, S Proc Natl Acad Sci U S A Research Article The stromelysins are members of a family of extracellular matrix metalloproteinases. These enzymes may erode the connective tissue in atherosclerotic plaques, leading to fissuring and acute thrombotic events. Cell-specific stromelysin expression in human atherosclerotic plaques was studied by in situ hybridization and immunocytochemistry. Sections were taken from nine coronary arteries: eight with well-established plaques and one normal. Unambiguous signals were seen in five plaques, two were inconclusive, and the remaining sample was negative, as was the normal coronary artery. Stromelysin mRNA transcripts were localized to isolated individual cells, some of which were smooth muscle, in the plaque cap, intima, and adventitia, but not the media. Expression was also seen in large clusters of macrophages that contained intracellular lipid deposits. The isolated expression of stromelysin by smooth muscle cells may reflect local connective tissue remodeling associated with growth and the formation of the plaque, whereas the more extensive expression associated with macrophages may be of greater pathological significance, contributing to the destabilization of the extracellular matrix and eventual plaque rupture. 1991-09-15 /pmc/articles/PMC52465/ /pubmed/1896464 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Henney, A M
Wakeley, P R
Davies, M J
Foster, K
Hembry, R
Murphy, G
Humphries, S
Localization of stromelysin gene expression in atherosclerotic plaques by in situ hybridization.
description The stromelysins are members of a family of extracellular matrix metalloproteinases. These enzymes may erode the connective tissue in atherosclerotic plaques, leading to fissuring and acute thrombotic events. Cell-specific stromelysin expression in human atherosclerotic plaques was studied by in situ hybridization and immunocytochemistry. Sections were taken from nine coronary arteries: eight with well-established plaques and one normal. Unambiguous signals were seen in five plaques, two were inconclusive, and the remaining sample was negative, as was the normal coronary artery. Stromelysin mRNA transcripts were localized to isolated individual cells, some of which were smooth muscle, in the plaque cap, intima, and adventitia, but not the media. Expression was also seen in large clusters of macrophages that contained intracellular lipid deposits. The isolated expression of stromelysin by smooth muscle cells may reflect local connective tissue remodeling associated with growth and the formation of the plaque, whereas the more extensive expression associated with macrophages may be of greater pathological significance, contributing to the destabilization of the extracellular matrix and eventual plaque rupture.
author Henney, A M
Wakeley, P R
Davies, M J
Foster, K
Hembry, R
Murphy, G
Humphries, S
author_facet Henney, A M
Wakeley, P R
Davies, M J
Foster, K
Hembry, R
Murphy, G
Humphries, S
author_sort Henney, A M
title Localization of stromelysin gene expression in atherosclerotic plaques by in situ hybridization.
title_short Localization of stromelysin gene expression in atherosclerotic plaques by in situ hybridization.
title_full Localization of stromelysin gene expression in atherosclerotic plaques by in situ hybridization.
title_fullStr Localization of stromelysin gene expression in atherosclerotic plaques by in situ hybridization.
title_full_unstemmed Localization of stromelysin gene expression in atherosclerotic plaques by in situ hybridization.
title_sort localization of stromelysin gene expression in atherosclerotic plaques by in situ hybridization.
publishDate 1991
url https://ncbi.nlm.nih.gov/pmc/articles/PMC52465/
https://ncbi.nlm.nih.gov/pubmed/1896464
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