Transcription Impacts the Efficiency of mRNA Translation via Co-transcriptional N6-adenosine Methylation

Transcription and translation are two main pillars of gene expression. Due to the different timings, spots of action, and mechanisms of regulation, these processes are mainly regarded as distinct and generally uncoupled, despite serving a common purpose. Here, we sought for a possible connection bet...

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Publicat: Cell Press 2017
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5388891/
https://ncbi.nlm.nih.gov/pubmed/28388414
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2017.03.031
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id pubmed-5388891
record_format dspace
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Article
spellingShingle Article
Transcription Impacts the Efficiency of mRNA Translation via Co-transcriptional N6-adenosine Methylation
topic_facet Article
description Transcription and translation are two main pillars of gene expression. Due to the different timings, spots of action, and mechanisms of regulation, these processes are mainly regarded as distinct and generally uncoupled, despite serving a common purpose. Here, we sought for a possible connection between transcription and translation. Employing an unbiased screen of multiple human promoters, we identified a positive effect of TATA box on translation and a general coupling between mRNA expression and translational efficiency. Using a CRISPR-Cas9-mediated approach, genome-wide analyses, and in vitro experiments, we show that the rate of transcription regulates the efficiency of translation. Furthermore, we demonstrate that m(6)A modification of mRNAs is co-transcriptional and depends upon the dynamics of the transcribing RNAPII. Suboptimal transcription rates lead to elevated m(6)A content, which may result in reduced translation. This study uncovers a general and widespread link between transcription and translation that is governed by epigenetic modification of mRNAs.
author_sort Slobodin, Boris
title Transcription Impacts the Efficiency of mRNA Translation via Co-transcriptional N6-adenosine Methylation
title_short Transcription Impacts the Efficiency of mRNA Translation via Co-transcriptional N6-adenosine Methylation
title_full Transcription Impacts the Efficiency of mRNA Translation via Co-transcriptional N6-adenosine Methylation
title_fullStr Transcription Impacts the Efficiency of mRNA Translation via Co-transcriptional N6-adenosine Methylation
title_full_unstemmed Transcription Impacts the Efficiency of mRNA Translation via Co-transcriptional N6-adenosine Methylation
title_sort transcription impacts the efficiency of mrna translation via co-transcriptional n6-adenosine methylation
publisher Cell Press
publisher_facet Cell Press
publishDate 2017
url https://ncbi.nlm.nih.gov/pmc/articles/PMC5388891/
https://ncbi.nlm.nih.gov/pubmed/28388414
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2017.03.031
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