Specific binding of radioiodinated granulocyte-macrophage colony-stimulating factor to hemopoietic cells.

The hemopoietic growth factor granulocyte-macrophage colony-stimulating factor, GM-CSF, specifically controls the production of granulocytes and macrophages. This report describes the binding of biologically-active 125I-labeled murine GM-CSF to a range of hemopoietic cells. Specific binding was rest...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Walker, F, Burgess, A W
Formato: Artigo
Idioma:English
Publicado em: 1985
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC554282/
https://ncbi.nlm.nih.gov/pubmed/2990915
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
id pubmed-554282
record_format dspace
spelling pubmed-5542822005-04-27 Specific binding of radioiodinated granulocyte-macrophage colony-stimulating factor to hemopoietic cells. Walker, F Burgess, A W EMBO J Research Article The hemopoietic growth factor granulocyte-macrophage colony-stimulating factor, GM-CSF, specifically controls the production of granulocytes and macrophages. This report describes the binding of biologically-active 125I-labeled murine GM-CSF to a range of hemopoietic cells. Specific binding was restricted to murine cells and neither rat nor human bone marrow cells appeared to have surface receptors for 125I-labeled GM-CSF. 125I-Labeled GM-CSF only appeared to bind specifically to cells in the myelomonocytic lineage. The binding of 125I-labeled GM-CSF to both bone marrow cells and WEHI-3B(D+) was rapid (50% maximum binding was attained within 5 min at both 20 degrees C and 37 degrees C). Unlabeled GM-CSF was the only polypeptide hormone which completely inhibited the binding of 125I-labeled GM-CSF to bone marrow cells, however, multi-CSF (also called IL-3) and G-CSF partially reduced the binding of 125I-labeled GM-CSF to bone marrow cells. Interestingly, the binding of 125I-labeled GM-CSF to a myelomonocytic cell line, WEHI-3B(D+), was inhibited by unlabeled GM-CSF but not by multi-CSF or G-CSF. Scatchard analysis of the binding of 125I-labeled GM-CSF to WEHI-3B(D+) cells, bone marrow cells and peritoneal neutrophils indicated that there were two classes of binding sites: one of high affinity (Kd1 = 20 pM) and one of low affinity (Kd2 = 0.8-1.2 nM). Multi-CSF only inhibited the binding of 125I-labeled GM-CSF to the high affinity receptor on bone marrow cells: this inhibition appeared to be a result of down regulation or modification of the GM-CSF receptor.(ABSTRACT TRUNCATED AT 250 WORDS) 1985-04 /pmc/articles/PMC554282/ /pubmed/2990915 Text en
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Research Article
spellingShingle Research Article
Walker, F
Burgess, A W
Specific binding of radioiodinated granulocyte-macrophage colony-stimulating factor to hemopoietic cells.
description The hemopoietic growth factor granulocyte-macrophage colony-stimulating factor, GM-CSF, specifically controls the production of granulocytes and macrophages. This report describes the binding of biologically-active 125I-labeled murine GM-CSF to a range of hemopoietic cells. Specific binding was restricted to murine cells and neither rat nor human bone marrow cells appeared to have surface receptors for 125I-labeled GM-CSF. 125I-Labeled GM-CSF only appeared to bind specifically to cells in the myelomonocytic lineage. The binding of 125I-labeled GM-CSF to both bone marrow cells and WEHI-3B(D+) was rapid (50% maximum binding was attained within 5 min at both 20 degrees C and 37 degrees C). Unlabeled GM-CSF was the only polypeptide hormone which completely inhibited the binding of 125I-labeled GM-CSF to bone marrow cells, however, multi-CSF (also called IL-3) and G-CSF partially reduced the binding of 125I-labeled GM-CSF to bone marrow cells. Interestingly, the binding of 125I-labeled GM-CSF to a myelomonocytic cell line, WEHI-3B(D+), was inhibited by unlabeled GM-CSF but not by multi-CSF or G-CSF. Scatchard analysis of the binding of 125I-labeled GM-CSF to WEHI-3B(D+) cells, bone marrow cells and peritoneal neutrophils indicated that there were two classes of binding sites: one of high affinity (Kd1 = 20 pM) and one of low affinity (Kd2 = 0.8-1.2 nM). Multi-CSF only inhibited the binding of 125I-labeled GM-CSF to the high affinity receptor on bone marrow cells: this inhibition appeared to be a result of down regulation or modification of the GM-CSF receptor.(ABSTRACT TRUNCATED AT 250 WORDS)
author Walker, F
Burgess, A W
author_facet Walker, F
Burgess, A W
author_sort Walker, F
title Specific binding of radioiodinated granulocyte-macrophage colony-stimulating factor to hemopoietic cells.
title_short Specific binding of radioiodinated granulocyte-macrophage colony-stimulating factor to hemopoietic cells.
title_full Specific binding of radioiodinated granulocyte-macrophage colony-stimulating factor to hemopoietic cells.
title_fullStr Specific binding of radioiodinated granulocyte-macrophage colony-stimulating factor to hemopoietic cells.
title_full_unstemmed Specific binding of radioiodinated granulocyte-macrophage colony-stimulating factor to hemopoietic cells.
title_sort specific binding of radioiodinated granulocyte-macrophage colony-stimulating factor to hemopoietic cells.
publishDate 1985
url https://ncbi.nlm.nih.gov/pmc/articles/PMC554282/
https://ncbi.nlm.nih.gov/pubmed/2990915
_version_ 1760255110265438208

Registos relacionados