Завантаження...
Partitioning of a polymer into a nanoscopic protein pore obeys a simple scaling law
The dependence of the rate on polymer mass was examined for the reaction of four sulfhydryl-directed poly(ethylene glycol) reagents with cysteine residues located in the lumen of the staphylococcal α-hemolysin pore. The logarithms of the apparent rate constants for a particular site in the lumen wer...
Збережено в:
| Автори: | , |
|---|---|
| Формат: | Стаття |
| Мова: | en |
| Опубліковано: |
The National Academy of Sciences
2001
|
| Предмети: | |
| Онлайн доступ: | https://ncbi.nlm.nih.gov/pmc/articles/PMC56928/ https://ncbi.nlm.nih.gov/pubmed/11504913 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.181089798 |
| Теги: |
Додати тег
Немає тегів, Будьте першим, хто поставить тег для цього запису!
|
| id |
pubmed-56928 |
|---|---|
| record_format |
dspace |
| spelling |
pubmed-569282001-10-01 Partitioning of a polymer into a nanoscopic protein pore obeys a simple scaling law Movileanu, Liviu Bayley, Hagan Proc Natl Acad Sci U S A Biological Sciences The dependence of the rate on polymer mass was examined for the reaction of four sulfhydryl-directed poly(ethylene glycol) reagents with cysteine residues located in the lumen of the staphylococcal α-hemolysin pore. The logarithms of the apparent rate constants for a particular site in the lumen were proportional to N, the number of repeat units in a polymer chain. The proportionality constant was −(a/D)(5/3), where a is the persistence length of the polymer (≈3.5Å) and D is the diameter of the pore. Despite some incongruencies with the assumptions of the derivation, the result suggests that the polymers partition into the lumen of the pore according to the simple scaling law of Daoud and de Gennes, c(pore)/c(solution) = exp(−N(a/D)(5/3)). Therefore, the measured reaction rates yield an estimate of the diameter of the pore and might be applied to determine the approximate dimensions of cavities within other similar proteins. The National Academy of Sciences 2001-08-28 2001-08-14 /pmc/articles/PMC56928/ /pubmed/11504913 http://dx.doi.org/10.1073/pnas.181089798 Text en Copyright © 2001, The National Academy of Sciences |
| institution |
US National Library of Medicine |
| collection |
PubMed Central |
| language |
en |
| format |
Article |
| topic |
Biological Sciences |
| spellingShingle |
Biological Sciences Movileanu, Liviu Bayley, Hagan Partitioning of a polymer into a nanoscopic protein pore obeys a simple scaling law |
| description |
The dependence of the rate on polymer mass was examined for the reaction of four sulfhydryl-directed poly(ethylene glycol) reagents with cysteine residues located in the lumen of the staphylococcal α-hemolysin pore. The logarithms of the apparent rate constants for a particular site in the lumen were proportional to N, the number of repeat units in a polymer chain. The proportionality constant was −(a/D)(5/3), where a is the persistence length of the polymer (≈3.5Å) and D is the diameter of the pore. Despite some incongruencies with the assumptions of the derivation, the result suggests that the polymers partition into the lumen of the pore according to the simple scaling law of Daoud and de Gennes, c(pore)/c(solution) = exp(−N(a/D)(5/3)). Therefore, the measured reaction rates yield an estimate of the diameter of the pore and might be applied to determine the approximate dimensions of cavities within other similar proteins. |
| author |
Movileanu, Liviu Bayley, Hagan |
| author_facet |
Movileanu, Liviu Bayley, Hagan |
| author_sort |
Movileanu, Liviu |
| title |
Partitioning of a polymer into a nanoscopic protein pore obeys a simple scaling law |
| title_short |
Partitioning of a polymer into a nanoscopic protein pore obeys a simple scaling law |
| title_full |
Partitioning of a polymer into a nanoscopic protein pore obeys a simple scaling law |
| title_fullStr |
Partitioning of a polymer into a nanoscopic protein pore obeys a simple scaling law |
| title_full_unstemmed |
Partitioning of a polymer into a nanoscopic protein pore obeys a simple scaling law |
| title_sort |
partitioning of a polymer into a nanoscopic protein pore obeys a simple scaling law |
| publisher |
The National Academy of Sciences |
| publisher_facet |
The National Academy of Sciences |
| publishDate |
2001 |
| url |
https://ncbi.nlm.nih.gov/pmc/articles/PMC56928/ https://ncbi.nlm.nih.gov/pubmed/11504913 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.181089798 |
| _version_ |
1758997240732450816 |