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Heavy-Metal Regulation of Thioredoxin Gene Expression in Chlamydomonas reinhardtii
Heavy metals are highly toxic compounds for cells. In this report we demonstrate that the expression of Chlamydomonas reinhardtii thioredoxins (TRX) m and h is induced by heavy metals. Upon exposure of the cells to Cd and Hg, a strong accumulation of both messengers was observed. Western-blot experi...
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American Society of Plant Physiologists
1999
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| Mynediad Ar-lein: | https://ncbi.nlm.nih.gov/pmc/articles/PMC59315/ https://ncbi.nlm.nih.gov/pubmed/10398712 |
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pubmed-593152001-11-02 Heavy-Metal Regulation of Thioredoxin Gene Expression in Chlamydomonas reinhardtii Lemaire, Stéphane Keryer, Eliane Stein, Mariana Schepens, Isabelle Issakidis-Bourguet, Emmanuelle Gérard-Hirne, Catherine Miginiac-Maslow, Myroslawa Jacquot, Jean-Pierre Plant Physiol Research Article Heavy metals are highly toxic compounds for cells. In this report we demonstrate that the expression of Chlamydomonas reinhardtii thioredoxins (TRX) m and h is induced by heavy metals. Upon exposure of the cells to Cd and Hg, a strong accumulation of both messengers was observed. Western-blot experiments revealed that among these two TRXs, only TRX h polypeptides accumulated in response to the toxic cations. A biochemical analysis indicated that heavy metals inhibit TRX activity, presumably by binding at the level of their active site. Sequence analysis of the C. reinhardtii TRX h promoter revealed the presence of cis-acting elements related to cadmium induction. The origins and purposes of this regulation are discussed. Our data suggest, for the first time to our knowledge, a possible implication of TRXs in defense mechanisms against heavy metals. American Society of Plant Physiologists 1999-07 /pmc/articles/PMC59315/ /pubmed/10398712 Text en |
| institution |
US National Library of Medicine |
| collection |
PubMed Central |
| language |
en |
| format |
Article |
| topic |
Research Article |
| spellingShingle |
Research Article Lemaire, Stéphane Keryer, Eliane Stein, Mariana Schepens, Isabelle Issakidis-Bourguet, Emmanuelle Gérard-Hirne, Catherine Miginiac-Maslow, Myroslawa Jacquot, Jean-Pierre Heavy-Metal Regulation of Thioredoxin Gene Expression in Chlamydomonas reinhardtii |
| description |
Heavy metals are highly toxic compounds for cells. In this report we demonstrate that the expression of Chlamydomonas reinhardtii thioredoxins (TRX) m and h is induced by heavy metals. Upon exposure of the cells to Cd and Hg, a strong accumulation of both messengers was observed. Western-blot experiments revealed that among these two TRXs, only TRX h polypeptides accumulated in response to the toxic cations. A biochemical analysis indicated that heavy metals inhibit TRX activity, presumably by binding at the level of their active site. Sequence analysis of the C. reinhardtii TRX h promoter revealed the presence of cis-acting elements related to cadmium induction. The origins and purposes of this regulation are discussed. Our data suggest, for the first time to our knowledge, a possible implication of TRXs in defense mechanisms against heavy metals. |
| author |
Lemaire, Stéphane Keryer, Eliane Stein, Mariana Schepens, Isabelle Issakidis-Bourguet, Emmanuelle Gérard-Hirne, Catherine Miginiac-Maslow, Myroslawa Jacquot, Jean-Pierre |
| author_facet |
Lemaire, Stéphane Keryer, Eliane Stein, Mariana Schepens, Isabelle Issakidis-Bourguet, Emmanuelle Gérard-Hirne, Catherine Miginiac-Maslow, Myroslawa Jacquot, Jean-Pierre |
| author_sort |
Lemaire, Stéphane |
| title |
Heavy-Metal Regulation of Thioredoxin Gene Expression in Chlamydomonas reinhardtii |
| title_short |
Heavy-Metal Regulation of Thioredoxin Gene Expression in Chlamydomonas reinhardtii |
| title_full |
Heavy-Metal Regulation of Thioredoxin Gene Expression in Chlamydomonas reinhardtii |
| title_fullStr |
Heavy-Metal Regulation of Thioredoxin Gene Expression in Chlamydomonas reinhardtii |
| title_full_unstemmed |
Heavy-Metal Regulation of Thioredoxin Gene Expression in Chlamydomonas reinhardtii |
| title_sort |
heavy-metal regulation of thioredoxin gene expression in chlamydomonas reinhardtii |
| publisher |
American Society of Plant Physiologists |
| publisher_facet |
American Society of Plant Physiologists |
| publishDate |
1999 |
| url |
https://ncbi.nlm.nih.gov/pmc/articles/PMC59315/ https://ncbi.nlm.nih.gov/pubmed/10398712 |
| _version_ |
1758997375454543872 |