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Isolation and Characterization of Polymorphic DNA from Entamoeba histolytica

An important gap in our understanding of the epidemiology of amebiasis is what determines the outcome of Entamoeba histolytica infections. To investigate the possible existence of invasive and noninvasive strains as one factor, the ability to differentiate individual isolates of E. histolytica is ne...

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Principais autores: Zaki, Mehreen, Clark, C. Graham, Langedijk, J. P. M., Middel, W. G. J., Meloen, R. H., Kramps, J. A., de Smit, J. A., Shin, Young Kyoo, Cai, Guang-Yun, Weinberg, Adriana, Leary, Jeffry J., Levin, Myron J., Halbur, P. G., Kasorndorkbua, C., Gilbert, C., Guenette, D., Potters, M. B., Purcell, R. H., Emerson, S. U., Toth, T. E., Meng, X. J., Townsend, Kirsty M., Boyce, John D., Chung, Jing Y., Frost, Alan J., Adler, Ben, Friis-Møller, Alice, Christensen, Jens Jørgen, Fussing, Vivian, Hesselbjerg, Annemarie, Christiansen, Jytte, Bruun, Brita, Harmsen, Dag, Singer, Christian, Rothgänger, Jörg, Tønjum, Tone, Sybren de Hoog, Gerrit, Shah, Haroun, Albert, Jürgen, Frosch, Matthias, Barrett, Sara L. Rassoulian, Cookson, Brad T., Carlson, LaDonna C., Bernard, Kathryn A., Coyle, Marie B., St-Germain, G., Laverdière, M., Pelletier, R., Bourgault, A.-M., Libman, M., Lemieux, C., Noël, G., Espinel-Ingroff, Ana, Ward, K. N., Turner, D. J., Parada, X. Couto, Thiruchelvam, A. D., Kellogg, James A., Bankert, David A., Withers, Gisela S., Sweimler, William, Kiehn, Timothy E., Pfyffer, Gaby E., Mansfield, Keith G., Lin, Kuei-Chin, Newman, Joseph, Schauer, David, MacKey, John, Lackner, Andrew A., Carville, Angela, Wu, Han-Chung, Huang, Yue-Ling, Chao, Ting-Ting, Jan, Jia-Tsrong, Huang, Jau-Ling, Chiang, Hsien-Yuan, King, Chwan-Chuen, Shaio, Men-Fang, Wilson, Michael L., Mirrett, Stanley, Meredith, Frances T., Weinstein, Melvin P., Scotto, Vincenzo, Reller, L. Barth, Berriatua, E., Ziluaga, I., Miguel-Virto, C., Uribarren, P., Juste, R., Laevens, S., Vandamme, P., Govan, J. R. W., Homer, K. A., Roberts, G., Byers, H. L., Tarelli, E., Whiley, R. A., Philpott-Howard, J., Beighton, D., House, Deborah, Wain, John, Ho, Vo A., Diep, To S., Chinh, Nguyen T., Bay, Phan V., Vinh, Ha, Duc, Minh, Parry, Christopher M., Dougan, Gordon, White, Nicholas J., Hien, Tran Tinh, Farrar, Jeremy J., Van Der Pol, Barbara, Ferrero, Dennis V., Buck-Barrington, Linda, Hook, Edward, Lenderman, Connie, Quinn, Thomas, Gaydos, Charlotte A., Lovchik, Judith, Schachter, Julius, Moncada, Jeanne, Hall, Geraldine, Tuohy, Marion J., Jones, Robert B., Koch, Walter H., Sullivan, Patrick S., Roberts, Charles, Francis, Kori, Downing, Robert, Mastro, Timothy D., Nkengasong, John, Hu, Dale, Masciotra, Silvina, Schable, Charles, Lal, Renu B., O'Brien, Katherine L., Bronsdon, Melinda A., Dagan, Ron, Yagupsky, Pablo, Janco, Jacob, Elliott, John, Whitney, Cynthia G., Yang, Yong-Hong, Robinson, Lisa-Gaye E., Schwartz, Benjamin, Carlone, George M., Tjitra, Emiliana, Suprianto, Sri, McBroom, James, Currie, Bart J., Anstey, Nicholas M., Hongmanee, Poonpilas, Stender, Henrik, Rasmussen, Ole F., Naylor, Matthew J., Harrison, Gavan A., Monckton, Robert P., McOrist, Steven, Lehrbach, Philip R., Deane, Elizabeth M., Lockman, Shahin, Sheppard, Jeffery D., Braden, Christopher R., Mwasekaga, Michael J., Woodley, Charles L., Kenyon, Thomas A., Binkin, Nancy J., Steinman, Michael, Montsho, Faustina, Kesupile-Reed, Matlhatso, Hirschfeldt, Colette, Notha, Malebogo, Moeti, Themba, Tappero, Jordan W., Chiou, Chien-Shun, Hsu, Wen-Bin, Wei, Hsiao-Lun, Chen, Jiann-Hwa, Tamada, Yukihiro, Nakaoka, Yuji, Nishimori, Kei, Doi, Akira, Kumaki, Takahiro, Uemura, Nobuko, Tanaka, Kiyoshi, Makino, Sou-Ichi, Sameshima, Toshiya, Akiba, Masato, Nakazawa, Muneo, Uchida, Ikuo, Caimi, Karina, Romano, Maria I., Alito, Alicia, Zumarraga, Martin, Bigi, Fabiana, Cataldi, Angel, Henry, Deborah A., Mahenthiralingam, Eshwar, Vandamme, Peter, Coenye, Tom, Speert, David P., Tortoli, Enrico, Nanetti, Anna, Piersimoni, Claudio, Cichero, Paola, Farina, Claudio, Mucignat, Giorgio, Scarparo, Claudio, Bartolini, Laura, Valentini, Roberta, Nista, Domenico, Gesu, Giampietro, Tosi, Cristiana Passerini, Crovatto, Marina, Brusarosco, Giuliana, Bishop, Ruth F., Masendycz, Paul J., Bugg, Helen C., Carlin, John B., Barnes, Graeme L., Benjamin, William H., Lok, Kerry H., Harris, Randall, Brook, Nancy, Bond, Lisa, Mulcahy, Donna, Robinson, Nancy, Pruitt, Virginia, Kirkpatrick, deNay P., Kimerling, Michael E., Dunlap, Nancy E., Westin, Lorelei, Miller, Carolyn, Vollmer, Dana, Canter, David, Radtkey, Ray, Nerenberg, Michael, O'Connell, James P., del Aguila, Carmen, Moura, Hercules, Fenoy, Soledad, Navajas, Raquel, Lopez-Velez, Rogelio, Li, Lixia, Xiao, Lihua, Leitch, Gordon J., da Silva, Alexandre, Pieniazek, Norman J., Lal, Altaf A., Visvesvara, Govinda S., Shukla, Sanjay K., Vevea, Dirk N., Frank, Daniel N., Pace, Norman R., Reed, Kurt D., Okuda, Katsuji, Ishihara, Kazuyuki, Nakagawa, Taneaki, Hirayama, Akihiko, Inayama, Yoshiyuki, Okuda, Kenji, Brambilla, Donald J., Granger, Suzanne, Jennings, Cheryl, Bremer, James W., La Seta Catamancio, Simona, De Pasquale, Maria Pia, Citterio, Paola, Kurtagic, Semir, Galli, Massimo, Rusconi, Stefano, Lünemann, Jan D., Zarmas, Silvia, Priem, Susanne, Franz, Juliane, Zschenderlein, Rolf, Aberer, Elisabeth, Klein, Rolf, Schouls, Leo, Burmester, Gerd R., Krause, Andreas, Gené, Josepa, Azón-Masoliver, Antoni, Guarro, Josep, De Febrer, Gabriel, Martínez, Angels, Grau, Cristina, Ortoneda, Montserrat, Ballester, Frederic, Amit, Zunika, Kaneshiro, Edna S., Saari, Marjut, Cheasty, Thomas, Leino, Kirsikka, Siitonen, Anja, Chiou, Christine C., McEllistrem, Mary Catherine, Bestetti, Arabella, Pierotti, Chiara, Terreni, Mariarosa, Zappa, Alessandra, Vago, Luca, Lazzarin, Adriano, Cinque, Paola, Hacek, Donna M., Bednarz, Patrice, Noskin, Gary A., Zembower, Teresa, Peterson, Lance R., Fryer, Jacqueline F. L., Griffiths, Paul D., Fishman, Jay A., Emery, Vincent C., Clark, Duncan A., Santos, Norma, Volotão, Eduardo M., Soares, Caroline C., Albuquerque, Maria Carolina M., da Silva, Fabiano M., de Carvalho, Tatiane R. B., Pereira, Cláudio F. A., Chizhikov, Vladimir, Hoshino, Yasutaka, Leclercq, Alexandre, Lambert, Bernard, Pierard, Denis, Mahillon, Jacques, Lee, Wee Gyo, Jernigan, John A., Rasheed, James K., Anderson, Gregory J., Tenover, Fred C.
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Publicado em: American Society for Microbiology 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC87847/
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spelling pubmed-878472002-05-02 Isolation and Characterization of Polymorphic DNA from Entamoeba histolytica Zaki, Mehreen Clark, C. Graham J Clin Microbiol Parasitology An important gap in our understanding of the epidemiology of amebiasis is what determines the outcome of Entamoeba histolytica infections. To investigate the possible existence of invasive and noninvasive strains as one factor, the ability to differentiate individual isolates of E. histolytica is necessary. Two new loci containing internal repeats, locus 1-2 and locus 5-6, have been isolated. Each contains a single repeat block with two types of related direct repeats arranged in tandem. Southern blot analysis suggests that both loci are multicopy and may themselves be arranged in tandem arrays. Three other previously reported, internally repetitive loci containing at least two repeat blocks each with one or more related repeat units were also investigated. PCR was used to study polymorphism at each of these loci, which was detected to various degrees in each case. Variation was seen in the total number of bands obtained per isolate and their sizes. Nucleotide sequence comparison of loci 1-2 and 5-6 in five axenic isolates revealed differences in the number of repeat units, which correlated with the observed PCR product size variation, and in repeat sequence. Use of multiple loci collectively allowed differentiation of a majority of the 13 isolates studied, and we believe that these loci have the potential to be used as polymorphic molecular markers for investigating the epidemiology of E. histolytica and the potential existence of genetically distinct invasive and noninvasive strains. American Society for Microbiology 2001-03 /pmc/articles/PMC87847/ /pubmed/11230401 http://dx.doi.org/10.1128/JCM.39.3.897-905.2001 Text en Copyright © 2001, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language English
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format Article
topic Parasitology
Clinical Veterinary Microbiology
Virology
Clinical Veterinary Microbiology
Bacteriology
Bacteriology
Bacteriology
Bacteriology
Mycology
Mycology
Virology
Mycobacteriology and Aerobic Actinomycetes
Clinical Veterinary Microbiology
Virology
Bacteriology
Clinical Veterinary Microbiology
Bacteriology
Epidemiology
Bacteriology
Virology
Bacteriology
Parasitology
Mycobacteriology and Aerobic Actinomycetes
Virology
Mycobacteriology and Aerobic Actinomycetes
Epidemiology
Bacteriology
Mycobacteriology and Aerobic Actinomycetes
Bacteriology
Mycobacteriology and Aerobic Actinomycetes
Epidemiology
Mycobacteriology and Aerobic Actinomycetes
Bacteriology
Parasitology
Bacteriology
Bacteriology
Virology
Virology
Virology
Bacteriology
Mycology
Parasitology
Epidemiology
Epidemiology
Virology
Bacteriology
Clinical Veterinary Microbiology
Virology
Bacteriology
Epidemiology
spellingShingle Parasitology
Clinical Veterinary Microbiology
Virology
Clinical Veterinary Microbiology
Bacteriology
Bacteriology
Bacteriology
Bacteriology
Mycology
Mycology
Virology
Mycobacteriology and Aerobic Actinomycetes
Clinical Veterinary Microbiology
Virology
Bacteriology
Clinical Veterinary Microbiology
Bacteriology
Epidemiology
Bacteriology
Virology
Bacteriology
Parasitology
Mycobacteriology and Aerobic Actinomycetes
Virology
Mycobacteriology and Aerobic Actinomycetes
Epidemiology
Bacteriology
Mycobacteriology and Aerobic Actinomycetes
Bacteriology
Mycobacteriology and Aerobic Actinomycetes
Epidemiology
Mycobacteriology and Aerobic Actinomycetes
Bacteriology
Parasitology
Bacteriology
Bacteriology
Virology
Virology
Virology
Bacteriology
Mycology
Parasitology
Epidemiology
Epidemiology
Virology
Bacteriology
Clinical Veterinary Microbiology
Virology
Bacteriology
Epidemiology
Zaki, Mehreen
Clark, C. Graham
Langedijk, J. P. M.
Middel, W. G. J.
Meloen, R. H.
Kramps, J. A.
de Smit, J. A.
Shin, Young Kyoo
Cai, Guang-Yun
Weinberg, Adriana
Leary, Jeffry J.
Levin, Myron J.
Halbur, P. G.
Kasorndorkbua, C.
Gilbert, C.
Guenette, D.
Potters, M. B.
Purcell, R. H.
Emerson, S. U.
Toth, T. E.
Meng, X. J.
Townsend, Kirsty M.
Boyce, John D.
Chung, Jing Y.
Frost, Alan J.
Adler, Ben
Friis-Møller, Alice
Christensen, Jens Jørgen
Fussing, Vivian
Hesselbjerg, Annemarie
Christiansen, Jytte
Bruun, Brita
Harmsen, Dag
Singer, Christian
Rothgänger, Jörg
Tønjum, Tone
Sybren de Hoog, Gerrit
Shah, Haroun
Albert, Jürgen
Frosch, Matthias
Barrett, Sara L. Rassoulian
Cookson, Brad T.
Carlson, LaDonna C.
Bernard, Kathryn A.
Coyle, Marie B.
St-Germain, G.
Laverdière, M.
Pelletier, R.
Bourgault, A.-M.
Libman, M.
Lemieux, C.
Noël, G.
Espinel-Ingroff, Ana
Ward, K. N.
Turner, D. J.
Parada, X. Couto
Thiruchelvam, A. D.
Kellogg, James A.
Bankert, David A.
Withers, Gisela S.
Sweimler, William
Kiehn, Timothy E.
Pfyffer, Gaby E.
Mansfield, Keith G.
Lin, Kuei-Chin
Newman, Joseph
Schauer, David
MacKey, John
Lackner, Andrew A.
Carville, Angela
Wu, Han-Chung
Huang, Yue-Ling
Chao, Ting-Ting
Jan, Jia-Tsrong
Huang, Jau-Ling
Chiang, Hsien-Yuan
King, Chwan-Chuen
Shaio, Men-Fang
Wilson, Michael L.
Mirrett, Stanley
Meredith, Frances T.
Weinstein, Melvin P.
Scotto, Vincenzo
Reller, L. Barth
Berriatua, E.
Ziluaga, I.
Miguel-Virto, C.
Uribarren, P.
Juste, R.
Laevens, S.
Vandamme, P.
Govan, J. R. W.
Homer, K. A.
Roberts, G.
Byers, H. L.
Tarelli, E.
Whiley, R. A.
Philpott-Howard, J.
Beighton, D.
House, Deborah
Wain, John
Ho, Vo A.
Diep, To S.
Chinh, Nguyen T.
Bay, Phan V.
Vinh, Ha
Duc, Minh
Parry, Christopher M.
Dougan, Gordon
White, Nicholas J.
Hien, Tran Tinh
Farrar, Jeremy J.
Van Der Pol, Barbara
Ferrero, Dennis V.
Buck-Barrington, Linda
Hook, Edward
Lenderman, Connie
Quinn, Thomas
Gaydos, Charlotte A.
Lovchik, Judith
Schachter, Julius
Moncada, Jeanne
Hall, Geraldine
Tuohy, Marion J.
Jones, Robert B.
Koch, Walter H.
Sullivan, Patrick S.
Roberts, Charles
Francis, Kori
Downing, Robert
Mastro, Timothy D.
Nkengasong, John
Hu, Dale
Masciotra, Silvina
Schable, Charles
Lal, Renu B.
O'Brien, Katherine L.
Bronsdon, Melinda A.
Dagan, Ron
Yagupsky, Pablo
Janco, Jacob
Elliott, John
Whitney, Cynthia G.
Yang, Yong-Hong
Robinson, Lisa-Gaye E.
Schwartz, Benjamin
Carlone, George M.
Tjitra, Emiliana
Suprianto, Sri
McBroom, James
Currie, Bart J.
Anstey, Nicholas M.
Hongmanee, Poonpilas
Stender, Henrik
Rasmussen, Ole F.
Naylor, Matthew J.
Harrison, Gavan A.
Monckton, Robert P.
McOrist, Steven
Lehrbach, Philip R.
Deane, Elizabeth M.
Lockman, Shahin
Sheppard, Jeffery D.
Braden, Christopher R.
Mwasekaga, Michael J.
Woodley, Charles L.
Kenyon, Thomas A.
Binkin, Nancy J.
Steinman, Michael
Montsho, Faustina
Kesupile-Reed, Matlhatso
Hirschfeldt, Colette
Notha, Malebogo
Moeti, Themba
Tappero, Jordan W.
Chiou, Chien-Shun
Hsu, Wen-Bin
Wei, Hsiao-Lun
Chen, Jiann-Hwa
Tamada, Yukihiro
Nakaoka, Yuji
Nishimori, Kei
Doi, Akira
Kumaki, Takahiro
Uemura, Nobuko
Tanaka, Kiyoshi
Makino, Sou-Ichi
Sameshima, Toshiya
Akiba, Masato
Nakazawa, Muneo
Uchida, Ikuo
Caimi, Karina
Romano, Maria I.
Alito, Alicia
Zumarraga, Martin
Bigi, Fabiana
Cataldi, Angel
Henry, Deborah A.
Mahenthiralingam, Eshwar
Vandamme, Peter
Coenye, Tom
Speert, David P.
Tortoli, Enrico
Nanetti, Anna
Piersimoni, Claudio
Cichero, Paola
Farina, Claudio
Mucignat, Giorgio
Scarparo, Claudio
Bartolini, Laura
Valentini, Roberta
Nista, Domenico
Gesu, Giampietro
Tosi, Cristiana Passerini
Crovatto, Marina
Brusarosco, Giuliana
Bishop, Ruth F.
Masendycz, Paul J.
Bugg, Helen C.
Carlin, John B.
Barnes, Graeme L.
Benjamin, William H.
Lok, Kerry H.
Harris, Randall
Brook, Nancy
Bond, Lisa
Mulcahy, Donna
Robinson, Nancy
Pruitt, Virginia
Kirkpatrick, deNay P.
Kimerling, Michael E.
Dunlap, Nancy E.
Westin, Lorelei
Miller, Carolyn
Vollmer, Dana
Canter, David
Radtkey, Ray
Nerenberg, Michael
O'Connell, James P.
del Aguila, Carmen
Moura, Hercules
Fenoy, Soledad
Navajas, Raquel
Lopez-Velez, Rogelio
Li, Lixia
Xiao, Lihua
Leitch, Gordon J.
da Silva, Alexandre
Pieniazek, Norman J.
Lal, Altaf A.
Visvesvara, Govinda S.
Shukla, Sanjay K.
Vevea, Dirk N.
Frank, Daniel N.
Pace, Norman R.
Reed, Kurt D.
Okuda, Katsuji
Ishihara, Kazuyuki
Nakagawa, Taneaki
Hirayama, Akihiko
Inayama, Yoshiyuki
Okuda, Kenji
Brambilla, Donald J.
Granger, Suzanne
Jennings, Cheryl
Bremer, James W.
Brambilla, Donald J.
Granger, Suzanne
Jennings, Cheryl
Bremer, James W.
La Seta Catamancio, Simona
De Pasquale, Maria Pia
Citterio, Paola
Kurtagic, Semir
Galli, Massimo
Rusconi, Stefano
Lünemann, Jan D.
Zarmas, Silvia
Priem, Susanne
Franz, Juliane
Zschenderlein, Rolf
Aberer, Elisabeth
Klein, Rolf
Schouls, Leo
Burmester, Gerd R.
Krause, Andreas
Gené, Josepa
Azón-Masoliver, Antoni
Guarro, Josep
De Febrer, Gabriel
Martínez, Angels
Grau, Cristina
Ortoneda, Montserrat
Ballester, Frederic
Amit, Zunika
Kaneshiro, Edna S.
Saari, Marjut
Cheasty, Thomas
Leino, Kirsikka
Siitonen, Anja
Chiou, Christine C.
McEllistrem, Mary Catherine
Bestetti, Arabella
Pierotti, Chiara
Terreni, Mariarosa
Zappa, Alessandra
Vago, Luca
Lazzarin, Adriano
Cinque, Paola
Hacek, Donna M.
Bednarz, Patrice
Noskin, Gary A.
Zembower, Teresa
Peterson, Lance R.
Fryer, Jacqueline F. L.
Griffiths, Paul D.
Fishman, Jay A.
Emery, Vincent C.
Clark, Duncan A.
Santos, Norma
Volotão, Eduardo M.
Soares, Caroline C.
Albuquerque, Maria Carolina M.
da Silva, Fabiano M.
de Carvalho, Tatiane R. B.
Pereira, Cláudio F. A.
Chizhikov, Vladimir
Hoshino, Yasutaka
Leclercq, Alexandre
Lambert, Bernard
Pierard, Denis
Mahillon, Jacques
Lee, Wee Gyo
Jernigan, John A.
Rasheed, James K.
Anderson, Gregory J.
Tenover, Fred C.
Isolation and Characterization of Polymorphic DNA from Entamoeba histolytica
description An important gap in our understanding of the epidemiology of amebiasis is what determines the outcome of Entamoeba histolytica infections. To investigate the possible existence of invasive and noninvasive strains as one factor, the ability to differentiate individual isolates of E. histolytica is necessary. Two new loci containing internal repeats, locus 1-2 and locus 5-6, have been isolated. Each contains a single repeat block with two types of related direct repeats arranged in tandem. Southern blot analysis suggests that both loci are multicopy and may themselves be arranged in tandem arrays. Three other previously reported, internally repetitive loci containing at least two repeat blocks each with one or more related repeat units were also investigated. PCR was used to study polymorphism at each of these loci, which was detected to various degrees in each case. Variation was seen in the total number of bands obtained per isolate and their sizes. Nucleotide sequence comparison of loci 1-2 and 5-6 in five axenic isolates revealed differences in the number of repeat units, which correlated with the observed PCR product size variation, and in repeat sequence. Use of multiple loci collectively allowed differentiation of a majority of the 13 isolates studied, and we believe that these loci have the potential to be used as polymorphic molecular markers for investigating the epidemiology of E. histolytica and the potential existence of genetically distinct invasive and noninvasive strains.
author Zaki, Mehreen
Clark, C. Graham
Langedijk, J. P. M.
Middel, W. G. J.
Meloen, R. H.
Kramps, J. A.
de Smit, J. A.
Shin, Young Kyoo
Cai, Guang-Yun
Weinberg, Adriana
Leary, Jeffry J.
Levin, Myron J.
Halbur, P. G.
Kasorndorkbua, C.
Gilbert, C.
Guenette, D.
Potters, M. B.
Purcell, R. H.
Emerson, S. U.
Toth, T. E.
Meng, X. J.
Townsend, Kirsty M.
Boyce, John D.
Chung, Jing Y.
Frost, Alan J.
Adler, Ben
Friis-Møller, Alice
Christensen, Jens Jørgen
Fussing, Vivian
Hesselbjerg, Annemarie
Christiansen, Jytte
Bruun, Brita
Harmsen, Dag
Singer, Christian
Rothgänger, Jörg
Tønjum, Tone
Sybren de Hoog, Gerrit
Shah, Haroun
Albert, Jürgen
Frosch, Matthias
Barrett, Sara L. Rassoulian
Cookson, Brad T.
Carlson, LaDonna C.
Bernard, Kathryn A.
Coyle, Marie B.
St-Germain, G.
Laverdière, M.
Pelletier, R.
Bourgault, A.-M.
Libman, M.
Lemieux, C.
Noël, G.
Espinel-Ingroff, Ana
Ward, K. N.
Turner, D. J.
Parada, X. Couto
Thiruchelvam, A. D.
Kellogg, James A.
Bankert, David A.
Withers, Gisela S.
Sweimler, William
Kiehn, Timothy E.
Pfyffer, Gaby E.
Mansfield, Keith G.
Lin, Kuei-Chin
Newman, Joseph
Schauer, David
MacKey, John
Lackner, Andrew A.
Carville, Angela
Wu, Han-Chung
Huang, Yue-Ling
Chao, Ting-Ting
Jan, Jia-Tsrong
Huang, Jau-Ling
Chiang, Hsien-Yuan
King, Chwan-Chuen
Shaio, Men-Fang
Wilson, Michael L.
Mirrett, Stanley
Meredith, Frances T.
Weinstein, Melvin P.
Scotto, Vincenzo
Reller, L. Barth
Berriatua, E.
Ziluaga, I.
Miguel-Virto, C.
Uribarren, P.
Juste, R.
Laevens, S.
Vandamme, P.
Govan, J. R. W.
Homer, K. A.
Roberts, G.
Byers, H. L.
Tarelli, E.
Whiley, R. A.
Philpott-Howard, J.
Beighton, D.
House, Deborah
Wain, John
Ho, Vo A.
Diep, To S.
Chinh, Nguyen T.
Bay, Phan V.
Vinh, Ha
Duc, Minh
Parry, Christopher M.
Dougan, Gordon
White, Nicholas J.
Hien, Tran Tinh
Farrar, Jeremy J.
Van Der Pol, Barbara
Ferrero, Dennis V.
Buck-Barrington, Linda
Hook, Edward
Lenderman, Connie
Quinn, Thomas
Gaydos, Charlotte A.
Lovchik, Judith
Schachter, Julius
Moncada, Jeanne
Hall, Geraldine
Tuohy, Marion J.
Jones, Robert B.
Koch, Walter H.
Sullivan, Patrick S.
Roberts, Charles
Francis, Kori
Downing, Robert
Mastro, Timothy D.
Nkengasong, John
Hu, Dale
Masciotra, Silvina
Schable, Charles
Lal, Renu B.
O'Brien, Katherine L.
Bronsdon, Melinda A.
Dagan, Ron
Yagupsky, Pablo
Janco, Jacob
Elliott, John
Whitney, Cynthia G.
Yang, Yong-Hong
Robinson, Lisa-Gaye E.
Schwartz, Benjamin
Carlone, George M.
Tjitra, Emiliana
Suprianto, Sri
McBroom, James
Currie, Bart J.
Anstey, Nicholas M.
Hongmanee, Poonpilas
Stender, Henrik
Rasmussen, Ole F.
Naylor, Matthew J.
Harrison, Gavan A.
Monckton, Robert P.
McOrist, Steven
Lehrbach, Philip R.
Deane, Elizabeth M.
Lockman, Shahin
Sheppard, Jeffery D.
Braden, Christopher R.
Mwasekaga, Michael J.
Woodley, Charles L.
Kenyon, Thomas A.
Binkin, Nancy J.
Steinman, Michael
Montsho, Faustina
Kesupile-Reed, Matlhatso
Hirschfeldt, Colette
Notha, Malebogo
Moeti, Themba
Tappero, Jordan W.
Chiou, Chien-Shun
Hsu, Wen-Bin
Wei, Hsiao-Lun
Chen, Jiann-Hwa
Tamada, Yukihiro
Nakaoka, Yuji
Nishimori, Kei
Doi, Akira
Kumaki, Takahiro
Uemura, Nobuko
Tanaka, Kiyoshi
Makino, Sou-Ichi
Sameshima, Toshiya
Akiba, Masato
Nakazawa, Muneo
Uchida, Ikuo
Caimi, Karina
Romano, Maria I.
Alito, Alicia
Zumarraga, Martin
Bigi, Fabiana
Cataldi, Angel
Henry, Deborah A.
Mahenthiralingam, Eshwar
Vandamme, Peter
Coenye, Tom
Speert, David P.
Tortoli, Enrico
Nanetti, Anna
Piersimoni, Claudio
Cichero, Paola
Farina, Claudio
Mucignat, Giorgio
Scarparo, Claudio
Bartolini, Laura
Valentini, Roberta
Nista, Domenico
Gesu, Giampietro
Tosi, Cristiana Passerini
Crovatto, Marina
Brusarosco, Giuliana
Bishop, Ruth F.
Masendycz, Paul J.
Bugg, Helen C.
Carlin, John B.
Barnes, Graeme L.
Benjamin, William H.
Lok, Kerry H.
Harris, Randall
Brook, Nancy
Bond, Lisa
Mulcahy, Donna
Robinson, Nancy
Pruitt, Virginia
Kirkpatrick, deNay P.
Kimerling, Michael E.
Dunlap, Nancy E.
Westin, Lorelei
Miller, Carolyn
Vollmer, Dana
Canter, David
Radtkey, Ray
Nerenberg, Michael
O'Connell, James P.
del Aguila, Carmen
Moura, Hercules
Fenoy, Soledad
Navajas, Raquel
Lopez-Velez, Rogelio
Li, Lixia
Xiao, Lihua
Leitch, Gordon J.
da Silva, Alexandre
Pieniazek, Norman J.
Lal, Altaf A.
Visvesvara, Govinda S.
Shukla, Sanjay K.
Vevea, Dirk N.
Frank, Daniel N.
Pace, Norman R.
Reed, Kurt D.
Okuda, Katsuji
Ishihara, Kazuyuki
Nakagawa, Taneaki
Hirayama, Akihiko
Inayama, Yoshiyuki
Okuda, Kenji
Brambilla, Donald J.
Granger, Suzanne
Jennings, Cheryl
Bremer, James W.
Brambilla, Donald J.
Granger, Suzanne
Jennings, Cheryl
Bremer, James W.
La Seta Catamancio, Simona
De Pasquale, Maria Pia
Citterio, Paola
Kurtagic, Semir
Galli, Massimo
Rusconi, Stefano
Lünemann, Jan D.
Zarmas, Silvia
Priem, Susanne
Franz, Juliane
Zschenderlein, Rolf
Aberer, Elisabeth
Klein, Rolf
Schouls, Leo
Burmester, Gerd R.
Krause, Andreas
Gené, Josepa
Azón-Masoliver, Antoni
Guarro, Josep
De Febrer, Gabriel
Martínez, Angels
Grau, Cristina
Ortoneda, Montserrat
Ballester, Frederic
Amit, Zunika
Kaneshiro, Edna S.
Saari, Marjut
Cheasty, Thomas
Leino, Kirsikka
Siitonen, Anja
Chiou, Christine C.
McEllistrem, Mary Catherine
Bestetti, Arabella
Pierotti, Chiara
Terreni, Mariarosa
Zappa, Alessandra
Vago, Luca
Lazzarin, Adriano
Cinque, Paola
Hacek, Donna M.
Bednarz, Patrice
Noskin, Gary A.
Zembower, Teresa
Peterson, Lance R.
Fryer, Jacqueline F. L.
Griffiths, Paul D.
Fishman, Jay A.
Emery, Vincent C.
Clark, Duncan A.
Santos, Norma
Volotão, Eduardo M.
Soares, Caroline C.
Albuquerque, Maria Carolina M.
da Silva, Fabiano M.
de Carvalho, Tatiane R. B.
Pereira, Cláudio F. A.
Chizhikov, Vladimir
Hoshino, Yasutaka
Leclercq, Alexandre
Lambert, Bernard
Pierard, Denis
Mahillon, Jacques
Lee, Wee Gyo
Jernigan, John A.
Rasheed, James K.
Anderson, Gregory J.
Tenover, Fred C.
author_facet Zaki, Mehreen
Clark, C. Graham
Langedijk, J. P. M.
Middel, W. G. J.
Meloen, R. H.
Kramps, J. A.
de Smit, J. A.
Shin, Young Kyoo
Cai, Guang-Yun
Weinberg, Adriana
Leary, Jeffry J.
Levin, Myron J.
Halbur, P. G.
Kasorndorkbua, C.
Gilbert, C.
Guenette, D.
Potters, M. B.
Purcell, R. H.
Emerson, S. U.
Toth, T. E.
Meng, X. J.
Townsend, Kirsty M.
Boyce, John D.
Chung, Jing Y.
Frost, Alan J.
Adler, Ben
Friis-Møller, Alice
Christensen, Jens Jørgen
Fussing, Vivian
Hesselbjerg, Annemarie
Christiansen, Jytte
Bruun, Brita
Harmsen, Dag
Singer, Christian
Rothgänger, Jörg
Tønjum, Tone
Sybren de Hoog, Gerrit
Shah, Haroun
Albert, Jürgen
Frosch, Matthias
Barrett, Sara L. Rassoulian
Cookson, Brad T.
Carlson, LaDonna C.
Bernard, Kathryn A.
Coyle, Marie B.
St-Germain, G.
Laverdière, M.
Pelletier, R.
Bourgault, A.-M.
Libman, M.
Lemieux, C.
Noël, G.
Espinel-Ingroff, Ana
Ward, K. N.
Turner, D. J.
Parada, X. Couto
Thiruchelvam, A. D.
Kellogg, James A.
Bankert, David A.
Withers, Gisela S.
Sweimler, William
Kiehn, Timothy E.
Pfyffer, Gaby E.
Mansfield, Keith G.
Lin, Kuei-Chin
Newman, Joseph
Schauer, David
MacKey, John
Lackner, Andrew A.
Carville, Angela
Wu, Han-Chung
Huang, Yue-Ling
Chao, Ting-Ting
Jan, Jia-Tsrong
Huang, Jau-Ling
Chiang, Hsien-Yuan
King, Chwan-Chuen
Shaio, Men-Fang
Wilson, Michael L.
Mirrett, Stanley
Meredith, Frances T.
Weinstein, Melvin P.
Scotto, Vincenzo
Reller, L. Barth
Berriatua, E.
Ziluaga, I.
Miguel-Virto, C.
Uribarren, P.
Juste, R.
Laevens, S.
Vandamme, P.
Govan, J. R. W.
Homer, K. A.
Roberts, G.
Byers, H. L.
Tarelli, E.
Whiley, R. A.
Philpott-Howard, J.
Beighton, D.
House, Deborah
Wain, John
Ho, Vo A.
Diep, To S.
Chinh, Nguyen T.
Bay, Phan V.
Vinh, Ha
Duc, Minh
Parry, Christopher M.
Dougan, Gordon
White, Nicholas J.
Hien, Tran Tinh
Farrar, Jeremy J.
Van Der Pol, Barbara
Ferrero, Dennis V.
Buck-Barrington, Linda
Hook, Edward
Lenderman, Connie
Quinn, Thomas
Gaydos, Charlotte A.
Lovchik, Judith
Schachter, Julius
Moncada, Jeanne
Hall, Geraldine
Tuohy, Marion J.
Jones, Robert B.
Koch, Walter H.
Sullivan, Patrick S.
Roberts, Charles
Francis, Kori
Downing, Robert
Mastro, Timothy D.
Nkengasong, John
Hu, Dale
Masciotra, Silvina
Schable, Charles
Lal, Renu B.
O'Brien, Katherine L.
Bronsdon, Melinda A.
Dagan, Ron
Yagupsky, Pablo
Janco, Jacob
Elliott, John
Whitney, Cynthia G.
Yang, Yong-Hong
Robinson, Lisa-Gaye E.
Schwartz, Benjamin
Carlone, George M.
Tjitra, Emiliana
Suprianto, Sri
McBroom, James
Currie, Bart J.
Anstey, Nicholas M.
Hongmanee, Poonpilas
Stender, Henrik
Rasmussen, Ole F.
Naylor, Matthew J.
Harrison, Gavan A.
Monckton, Robert P.
McOrist, Steven
Lehrbach, Philip R.
Deane, Elizabeth M.
Lockman, Shahin
Sheppard, Jeffery D.
Braden, Christopher R.
Mwasekaga, Michael J.
Woodley, Charles L.
Kenyon, Thomas A.
Binkin, Nancy J.
Steinman, Michael
Montsho, Faustina
Kesupile-Reed, Matlhatso
Hirschfeldt, Colette
Notha, Malebogo
Moeti, Themba
Tappero, Jordan W.
Chiou, Chien-Shun
Hsu, Wen-Bin
Wei, Hsiao-Lun
Chen, Jiann-Hwa
Tamada, Yukihiro
Nakaoka, Yuji
Nishimori, Kei
Doi, Akira
Kumaki, Takahiro
Uemura, Nobuko
Tanaka, Kiyoshi
Makino, Sou-Ichi
Sameshima, Toshiya
Akiba, Masato
Nakazawa, Muneo
Uchida, Ikuo
Caimi, Karina
Romano, Maria I.
Alito, Alicia
Zumarraga, Martin
Bigi, Fabiana
Cataldi, Angel
Henry, Deborah A.
Mahenthiralingam, Eshwar
Vandamme, Peter
Coenye, Tom
Speert, David P.
Tortoli, Enrico
Nanetti, Anna
Piersimoni, Claudio
Cichero, Paola
Farina, Claudio
Mucignat, Giorgio
Scarparo, Claudio
Bartolini, Laura
Valentini, Roberta
Nista, Domenico
Gesu, Giampietro
Tosi, Cristiana Passerini
Crovatto, Marina
Brusarosco, Giuliana
Bishop, Ruth F.
Masendycz, Paul J.
Bugg, Helen C.
Carlin, John B.
Barnes, Graeme L.
Benjamin, William H.
Lok, Kerry H.
Harris, Randall
Brook, Nancy
Bond, Lisa
Mulcahy, Donna
Robinson, Nancy
Pruitt, Virginia
Kirkpatrick, deNay P.
Kimerling, Michael E.
Dunlap, Nancy E.
Westin, Lorelei
Miller, Carolyn
Vollmer, Dana
Canter, David
Radtkey, Ray
Nerenberg, Michael
O'Connell, James P.
del Aguila, Carmen
Moura, Hercules
Fenoy, Soledad
Navajas, Raquel
Lopez-Velez, Rogelio
Li, Lixia
Xiao, Lihua
Leitch, Gordon J.
da Silva, Alexandre
Pieniazek, Norman J.
Lal, Altaf A.
Visvesvara, Govinda S.
Shukla, Sanjay K.
Vevea, Dirk N.
Frank, Daniel N.
Pace, Norman R.
Reed, Kurt D.
Okuda, Katsuji
Ishihara, Kazuyuki
Nakagawa, Taneaki
Hirayama, Akihiko
Inayama, Yoshiyuki
Okuda, Kenji
Brambilla, Donald J.
Granger, Suzanne
Jennings, Cheryl
Bremer, James W.
Brambilla, Donald J.
Granger, Suzanne
Jennings, Cheryl
Bremer, James W.
La Seta Catamancio, Simona
De Pasquale, Maria Pia
Citterio, Paola
Kurtagic, Semir
Galli, Massimo
Rusconi, Stefano
Lünemann, Jan D.
Zarmas, Silvia
Priem, Susanne
Franz, Juliane
Zschenderlein, Rolf
Aberer, Elisabeth
Klein, Rolf
Schouls, Leo
Burmester, Gerd R.
Krause, Andreas
Gené, Josepa
Azón-Masoliver, Antoni
Guarro, Josep
De Febrer, Gabriel
Martínez, Angels
Grau, Cristina
Ortoneda, Montserrat
Ballester, Frederic
Amit, Zunika
Kaneshiro, Edna S.
Saari, Marjut
Cheasty, Thomas
Leino, Kirsikka
Siitonen, Anja
Chiou, Christine C.
McEllistrem, Mary Catherine
Bestetti, Arabella
Pierotti, Chiara
Terreni, Mariarosa
Zappa, Alessandra
Vago, Luca
Lazzarin, Adriano
Cinque, Paola
Hacek, Donna M.
Bednarz, Patrice
Noskin, Gary A.
Zembower, Teresa
Peterson, Lance R.
Fryer, Jacqueline F. L.
Griffiths, Paul D.
Fishman, Jay A.
Emery, Vincent C.
Clark, Duncan A.
Santos, Norma
Volotão, Eduardo M.
Soares, Caroline C.
Albuquerque, Maria Carolina M.
da Silva, Fabiano M.
de Carvalho, Tatiane R. B.
Pereira, Cláudio F. A.
Chizhikov, Vladimir
Hoshino, Yasutaka
Leclercq, Alexandre
Lambert, Bernard
Pierard, Denis
Mahillon, Jacques
Lee, Wee Gyo
Jernigan, John A.
Rasheed, James K.
Anderson, Gregory J.
Tenover, Fred C.
author_sort Zaki, Mehreen
title Isolation and Characterization of Polymorphic DNA from Entamoeba histolytica
title_short Isolation and Characterization of Polymorphic DNA from Entamoeba histolytica
title_full Isolation and Characterization of Polymorphic DNA from Entamoeba histolytica
title_fullStr Isolation and Characterization of Polymorphic DNA from Entamoeba histolytica
title_full_unstemmed Isolation and Characterization of Polymorphic DNA from Entamoeba histolytica
title_sort isolation and characterization of polymorphic dna from entamoeba histolytica
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 2001
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