PCR-Based Detection and Identification of Burkholderia cepacia Complex Pathogens in Sputum from Cystic Fibrosis Patients

PCR amplification of the recA gene followed by restriction fragment length polymorphism (RFLP) analysis was investigated for the rapid detection and identification of Burkholderia cepacia complex genomovars directly from sputum. Successful amplification of the B. cepacia complex recA gene from cysti...

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Main Authors: McDowell, Andrew, Mahenthiralingam, Eshwar, Moore, John E., Dunbar, Kerstin E. A., Webb, A. Kevin, Dodd, Mary E., Martin, S. Lorraine, Millar, B. Cherie, Scott, Christopher J., Crowe, Mary, Elborn, J. Stuart
Formato: Artigo
Idioma:en
Publicado: American Society for Microbiology 2001
Assuntos:
Bacteriology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC88532/
https://ncbi.nlm.nih.gov/pubmed/11724828
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.12.4247-4255.2001
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https://ncbi.nlm.nih.gov/pmc/articles/PMC88532/
https://ncbi.nlm.nih.gov/pubmed/11724828
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.12.4247-4255.2001

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