Characterization of Mutations in the rpoB Gene in Naturally Rifampin-Resistant Rickettsia Species

Rickettsiae are gram-negative, obligately intracellular bacteria responsible for arthropod-borne spotted fevers and typhus. Experimental studies have delineated a cluster of naturally rifampin-resistant spotted fever group species. We sequenced the 4,122- to 4,125-bp RNA polymerase β-subunit-encodin...

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Main Authors: Drancourt, Michel, Raoult, Didier
Formato: Artigo
Idioma:en
Publicado em: American Society for Microbiology 1999
Assuntos:
Mechanisms of Resistance
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC89490/
https://ncbi.nlm.nih.gov/pubmed/10508014
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spelling pubmed-894902002-09-12 Characterization of Mutations in the rpoB Gene in Naturally Rifampin-Resistant Rickettsia Species Drancourt, Michel Raoult, Didier Antimicrob Agents Chemother Mechanisms of Resistance Rickettsiae are gram-negative, obligately intracellular bacteria responsible for arthropod-borne spotted fevers and typhus. Experimental studies have delineated a cluster of naturally rifampin-resistant spotted fever group species. We sequenced the 4,122- to 4,125-bp RNA polymerase β-subunit-encoding gene (rpoB) from typhus and spotted fever group representatives and obtained partial sequences for all naturally rifampin-resistant species. A single point mutation resulting in a phenylalanine-to-leucine change at position 973 of the Rickettsia conorii rpoB sequence and present in all the rifampin-resistant species was absent in all the rifampin-susceptible species. rpoB-based phylogenetic relationships among these rickettsial species yielded topologies which were in accordance with previously published phylogenies. American Society for Microbiology 1999-10 /pmc/articles/PMC89490/ /pubmed/10508014 Text en Copyright © 1999, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Mechanisms of Resistance
spellingShingle Mechanisms of Resistance
Drancourt, Michel
Raoult, Didier
Characterization of Mutations in the rpoB Gene in Naturally Rifampin-Resistant Rickettsia Species
description Rickettsiae are gram-negative, obligately intracellular bacteria responsible for arthropod-borne spotted fevers and typhus. Experimental studies have delineated a cluster of naturally rifampin-resistant spotted fever group species. We sequenced the 4,122- to 4,125-bp RNA polymerase β-subunit-encoding gene (rpoB) from typhus and spotted fever group representatives and obtained partial sequences for all naturally rifampin-resistant species. A single point mutation resulting in a phenylalanine-to-leucine change at position 973 of the Rickettsia conorii rpoB sequence and present in all the rifampin-resistant species was absent in all the rifampin-susceptible species. rpoB-based phylogenetic relationships among these rickettsial species yielded topologies which were in accordance with previously published phylogenies.
author Drancourt, Michel
Raoult, Didier
author_facet Drancourt, Michel
Raoult, Didier
author_sort Drancourt, Michel
title Characterization of Mutations in the rpoB Gene in Naturally Rifampin-Resistant Rickettsia Species
title_short Characterization of Mutations in the rpoB Gene in Naturally Rifampin-Resistant Rickettsia Species
title_full Characterization of Mutations in the rpoB Gene in Naturally Rifampin-Resistant Rickettsia Species
title_fullStr Characterization of Mutations in the rpoB Gene in Naturally Rifampin-Resistant Rickettsia Species
title_full_unstemmed Characterization of Mutations in the rpoB Gene in Naturally Rifampin-Resistant Rickettsia Species
title_sort characterization of mutations in the rpob gene in naturally rifampin-resistant rickettsia species
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 1999
url https://ncbi.nlm.nih.gov/pmc/articles/PMC89490/
https://ncbi.nlm.nih.gov/pubmed/10508014
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