Development of a PCR Assay for Detection of Yersinia ruckeri in Tissues of Inoculated and Naturally Infected Trout

A PCR-based method was developed for the specific detection of Yersinia ruckeri in tissues of inoculated trout and naturally infected trout. No amplification products were obtained with other yersiniae, bacterial fish pathogens, or phylogenetically related bacteria (n = 34). The sensitivity of PCR d...

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Main Authors: Gibello, A., Blanco, M. M., Moreno, M. A., Cutuli, M. T., Domenech, A., Domínguez, L., Fernández-Garayzábal, J. F.
Formato: Artigo
Idioma:en
Publicado em: American Society for Microbiology 1999
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC91030/
https://ncbi.nlm.nih.gov/pubmed/9872807
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spelling pubmed-910302002-09-12 Development of a PCR Assay for Detection of Yersinia ruckeri in Tissues of Inoculated and Naturally Infected Trout Gibello, A. Blanco, M. M. Moreno, M. A. Cutuli, M. T. Domenech, A. Domínguez, L. Fernández-Garayzábal, J. F. Appl Environ Microbiol Methods A PCR-based method was developed for the specific detection of Yersinia ruckeri in tissues of inoculated trout and naturally infected trout. No amplification products were obtained with other yersiniae, bacterial fish pathogens, or phylogenetically related bacteria (n = 34). The sensitivity of PCR detection was 60 to 65 bacterial cells per PCR tube, which was decreased to 10 to 20 cells by hybridization with a nonradioactive probe. The PCR assay proved to be as reliable as and faster than the conventional culture method for the detection of Y. ruckeri in infected trout tissues. American Society for Microbiology 1999-01 /pmc/articles/PMC91030/ /pubmed/9872807 Text en Copyright © 1999, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Methods
spellingShingle Methods
Gibello, A.
Blanco, M. M.
Moreno, M. A.
Cutuli, M. T.
Domenech, A.
Domínguez, L.
Fernández-Garayzábal, J. F.
Development of a PCR Assay for Detection of Yersinia ruckeri in Tissues of Inoculated and Naturally Infected Trout
description A PCR-based method was developed for the specific detection of Yersinia ruckeri in tissues of inoculated trout and naturally infected trout. No amplification products were obtained with other yersiniae, bacterial fish pathogens, or phylogenetically related bacteria (n = 34). The sensitivity of PCR detection was 60 to 65 bacterial cells per PCR tube, which was decreased to 10 to 20 cells by hybridization with a nonradioactive probe. The PCR assay proved to be as reliable as and faster than the conventional culture method for the detection of Y. ruckeri in infected trout tissues.
author Gibello, A.
Blanco, M. M.
Moreno, M. A.
Cutuli, M. T.
Domenech, A.
Domínguez, L.
Fernández-Garayzábal, J. F.
author_facet Gibello, A.
Blanco, M. M.
Moreno, M. A.
Cutuli, M. T.
Domenech, A.
Domínguez, L.
Fernández-Garayzábal, J. F.
author_sort Gibello, A.
title Development of a PCR Assay for Detection of Yersinia ruckeri in Tissues of Inoculated and Naturally Infected Trout
title_short Development of a PCR Assay for Detection of Yersinia ruckeri in Tissues of Inoculated and Naturally Infected Trout
title_full Development of a PCR Assay for Detection of Yersinia ruckeri in Tissues of Inoculated and Naturally Infected Trout
title_fullStr Development of a PCR Assay for Detection of Yersinia ruckeri in Tissues of Inoculated and Naturally Infected Trout
title_full_unstemmed Development of a PCR Assay for Detection of Yersinia ruckeri in Tissues of Inoculated and Naturally Infected Trout
title_sort development of a pcr assay for detection of yersinia ruckeri in tissues of inoculated and naturally infected trout
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 1999
url https://ncbi.nlm.nih.gov/pmc/articles/PMC91030/
https://ncbi.nlm.nih.gov/pubmed/9872807
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