Reductive Dechlorination of Tetrachloroethene to cis-1,2-Dichloroethene by a Thermophilic Anaerobic Enrichment Culture

Thermophilic anaerobic biodegradation of tetrachloroethene (PCE) was investigated with various inocula from geothermal and nongeothermal areas. Only polluted harbor sediment resulted in a stable enrichment culture that converted PCE via trichloroethene to cis-1,2-dichloroethene at the optimum temper...

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Autores principales: Kengen, Servé W. M., Breidenbach, Caroline G., Felske, Andreas, Stams, Alfons J. M., Schraa, Gosse, de Vos, Willem M.
Formato: Artículo
Lenguaje:en
Publicado: American Society for Microbiology 1999
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Environmental and Public Health Microbiology
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC91342/
https://ncbi.nlm.nih.gov/pubmed/10347007
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spelling pubmed-913422002-09-12 Reductive Dechlorination of Tetrachloroethene to cis-1,2-Dichloroethene by a Thermophilic Anaerobic Enrichment Culture Kengen, Servé W. M. Breidenbach, Caroline G. Felske, Andreas Stams, Alfons J. M. Schraa, Gosse de Vos, Willem M. Appl Environ Microbiol Environmental and Public Health Microbiology Thermophilic anaerobic biodegradation of tetrachloroethene (PCE) was investigated with various inocula from geothermal and nongeothermal areas. Only polluted harbor sediment resulted in a stable enrichment culture that converted PCE via trichloroethene to cis-1,2-dichloroethene at the optimum temperature of 60 to 65°C. After several transfers, methanogens were eliminated from the culture. Dechlorination was supported by lactate, pyruvate, fructose, fumarate, and malate as electron donor but not by H(2), formate, or acetate. Fumarate and l-malate led to the highest dechlorination rate. In the absence of PCE, fumarate was fermented to acetate, H(2), CO(2), and succinate. With PCE, less H(2) was formed, suggesting that PCE competed for the reducing equivalents leading to H(2). PCE dechlorination, apparently, was not outcompeted by fumarate as electron acceptor. At the optimum dissolved PCE concentration of ∼60 μM, a high dechlorination rate of 1.1 μmol h(−1) mg(−1) (dry weight) was found, which indicates that the dechlorination is not a cometabolic activity. Microscopic analysis of the fumarate-grown culture showed the dominance of a long thin rod. Molecular analysis, however, indicated the presence of two dominant species, both belonging to the low-G+C gram positives. The highest similarity was found with the genus Dehalobacter (90%), represented by the halorespiring organism Dehalobacter restrictus, and with the genus Desulfotomaculum (86%). American Society for Microbiology 1999-06 /pmc/articles/PMC91342/ /pubmed/10347007 Text en Copyright © 1999, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Environmental and Public Health Microbiology
spellingShingle Environmental and Public Health Microbiology
Kengen, Servé W. M.
Breidenbach, Caroline G.
Felske, Andreas
Stams, Alfons J. M.
Schraa, Gosse
de Vos, Willem M.
Reductive Dechlorination of Tetrachloroethene to cis-1,2-Dichloroethene by a Thermophilic Anaerobic Enrichment Culture
description Thermophilic anaerobic biodegradation of tetrachloroethene (PCE) was investigated with various inocula from geothermal and nongeothermal areas. Only polluted harbor sediment resulted in a stable enrichment culture that converted PCE via trichloroethene to cis-1,2-dichloroethene at the optimum temperature of 60 to 65°C. After several transfers, methanogens were eliminated from the culture. Dechlorination was supported by lactate, pyruvate, fructose, fumarate, and malate as electron donor but not by H(2), formate, or acetate. Fumarate and l-malate led to the highest dechlorination rate. In the absence of PCE, fumarate was fermented to acetate, H(2), CO(2), and succinate. With PCE, less H(2) was formed, suggesting that PCE competed for the reducing equivalents leading to H(2). PCE dechlorination, apparently, was not outcompeted by fumarate as electron acceptor. At the optimum dissolved PCE concentration of ∼60 μM, a high dechlorination rate of 1.1 μmol h(−1) mg(−1) (dry weight) was found, which indicates that the dechlorination is not a cometabolic activity. Microscopic analysis of the fumarate-grown culture showed the dominance of a long thin rod. Molecular analysis, however, indicated the presence of two dominant species, both belonging to the low-G+C gram positives. The highest similarity was found with the genus Dehalobacter (90%), represented by the halorespiring organism Dehalobacter restrictus, and with the genus Desulfotomaculum (86%).
author Kengen, Servé W. M.
Breidenbach, Caroline G.
Felske, Andreas
Stams, Alfons J. M.
Schraa, Gosse
de Vos, Willem M.
author_facet Kengen, Servé W. M.
Breidenbach, Caroline G.
Felske, Andreas
Stams, Alfons J. M.
Schraa, Gosse
de Vos, Willem M.
author_sort Kengen, Servé W. M.
title Reductive Dechlorination of Tetrachloroethene to cis-1,2-Dichloroethene by a Thermophilic Anaerobic Enrichment Culture
title_short Reductive Dechlorination of Tetrachloroethene to cis-1,2-Dichloroethene by a Thermophilic Anaerobic Enrichment Culture
title_full Reductive Dechlorination of Tetrachloroethene to cis-1,2-Dichloroethene by a Thermophilic Anaerobic Enrichment Culture
title_fullStr Reductive Dechlorination of Tetrachloroethene to cis-1,2-Dichloroethene by a Thermophilic Anaerobic Enrichment Culture
title_full_unstemmed Reductive Dechlorination of Tetrachloroethene to cis-1,2-Dichloroethene by a Thermophilic Anaerobic Enrichment Culture
title_sort reductive dechlorination of tetrachloroethene to cis-1,2-dichloroethene by a thermophilic anaerobic enrichment culture
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 1999
url https://ncbi.nlm.nih.gov/pmc/articles/PMC91342/
https://ncbi.nlm.nih.gov/pubmed/10347007
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