Bacillus thuringiensis Delta-Endotoxin Cry1C Domain III Can Function as a Specificity Determinant for Spodoptera exigua in Different, but Not All, Cry1-Cry1C Hybrids

In order to test our hypothesis that Bacillus thuringiensis delta-endotoxin Cry1Ca domain III functions as a determinant of specificity for Spodoptera exigua, regardless of the origins of domains I and II, we have constructed by cloning and in vivo recombination a collection of hybrid proteins conta...

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Main Authors: de Maagd, Ruud A., Weemen-Hendriks, Mieke, Stiekema, Willem, Bosch, Dirk
Formato: Artigo
Idioma:en
Publicado em: American Society for Microbiology 2000
Assuntos:
Invertebrate Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC92023/
https://ncbi.nlm.nih.gov/pubmed/10742242
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spelling pubmed-920232002-09-12 Bacillus thuringiensis Delta-Endotoxin Cry1C Domain III Can Function as a Specificity Determinant for Spodoptera exigua in Different, but Not All, Cry1-Cry1C Hybrids de Maagd, Ruud A. Weemen-Hendriks, Mieke Stiekema, Willem Bosch, Dirk Appl Environ Microbiol Invertebrate Microbiology In order to test our hypothesis that Bacillus thuringiensis delta-endotoxin Cry1Ca domain III functions as a determinant of specificity for Spodoptera exigua, regardless of the origins of domains I and II, we have constructed by cloning and in vivo recombination a collection of hybrid proteins containing domains I and II of various Cry1 toxins combined with domain III of Cry1Ca. Cry1Ab, Cry1Ac, Cry1Ba, Cry1Ea, and Cry1Fa all become more active against S. exigua when their domain III is replaced by (part of) that of Cry1Ca. This result shows that domain III of Cry1Ca is an important and versatile determinant of S. exigua specificity. The toxicity of the hybrids varied by a factor of 40, indicating that domain I and/or II modulate the activity as well. Cry1Da-Cry1Ca hybrids were an exception in that they were not significantly active against S. exigua or Manduca sexta, whereas both parental proteins were highly toxic. Incidentally, in a Cry1Ba-Cry1Ca hybrid, Cry1Ca domain III can also strongly increase toxicity for M. sexta. American Society for Microbiology 2000-04 /pmc/articles/PMC92023/ /pubmed/10742242 Text en Copyright © 2000, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Invertebrate Microbiology
spellingShingle Invertebrate Microbiology
de Maagd, Ruud A.
Weemen-Hendriks, Mieke
Stiekema, Willem
Bosch, Dirk
Bacillus thuringiensis Delta-Endotoxin Cry1C Domain III Can Function as a Specificity Determinant for Spodoptera exigua in Different, but Not All, Cry1-Cry1C Hybrids
description In order to test our hypothesis that Bacillus thuringiensis delta-endotoxin Cry1Ca domain III functions as a determinant of specificity for Spodoptera exigua, regardless of the origins of domains I and II, we have constructed by cloning and in vivo recombination a collection of hybrid proteins containing domains I and II of various Cry1 toxins combined with domain III of Cry1Ca. Cry1Ab, Cry1Ac, Cry1Ba, Cry1Ea, and Cry1Fa all become more active against S. exigua when their domain III is replaced by (part of) that of Cry1Ca. This result shows that domain III of Cry1Ca is an important and versatile determinant of S. exigua specificity. The toxicity of the hybrids varied by a factor of 40, indicating that domain I and/or II modulate the activity as well. Cry1Da-Cry1Ca hybrids were an exception in that they were not significantly active against S. exigua or Manduca sexta, whereas both parental proteins were highly toxic. Incidentally, in a Cry1Ba-Cry1Ca hybrid, Cry1Ca domain III can also strongly increase toxicity for M. sexta.
author de Maagd, Ruud A.
Weemen-Hendriks, Mieke
Stiekema, Willem
Bosch, Dirk
author_facet de Maagd, Ruud A.
Weemen-Hendriks, Mieke
Stiekema, Willem
Bosch, Dirk
author_sort de Maagd, Ruud A.
title Bacillus thuringiensis Delta-Endotoxin Cry1C Domain III Can Function as a Specificity Determinant for Spodoptera exigua in Different, but Not All, Cry1-Cry1C Hybrids
title_short Bacillus thuringiensis Delta-Endotoxin Cry1C Domain III Can Function as a Specificity Determinant for Spodoptera exigua in Different, but Not All, Cry1-Cry1C Hybrids
title_full Bacillus thuringiensis Delta-Endotoxin Cry1C Domain III Can Function as a Specificity Determinant for Spodoptera exigua in Different, but Not All, Cry1-Cry1C Hybrids
title_fullStr Bacillus thuringiensis Delta-Endotoxin Cry1C Domain III Can Function as a Specificity Determinant for Spodoptera exigua in Different, but Not All, Cry1-Cry1C Hybrids
title_full_unstemmed Bacillus thuringiensis Delta-Endotoxin Cry1C Domain III Can Function as a Specificity Determinant for Spodoptera exigua in Different, but Not All, Cry1-Cry1C Hybrids
title_sort bacillus thuringiensis delta-endotoxin cry1c domain iii can function as a specificity determinant for spodoptera exigua in different, but not all, cry1-cry1c hybrids
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 2000
url https://ncbi.nlm.nih.gov/pmc/articles/PMC92023/
https://ncbi.nlm.nih.gov/pubmed/10742242
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