Nitrile Hydratase and Amidase from Rhodococcus rhodochrous Hydrolyze Acrylic Fibers and Granular Polyacrylonitriles

Rhodococcus rhodochrous NCIMB 11216 produced nitrile hydratase (320 nkat mg of protein(−1)) and amidase activity (38.4 nkat mg of protein(−1)) when grown on a medium containing propionitrile. These enzymes were able to hydrolyze nitrile groups of both granular polyacrylonitriles (PAN) and acrylic fi...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Tauber, M. M., Cavaco-Paulo, A., Robra, K.-H., Gübitz, G. M.
Formato: Artigo
Idioma:en
Publicado em: American Society for Microbiology 2000
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC92034/
https://ncbi.nlm.nih.gov/pubmed/10742253
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
id pubmed-92034
record_format dspace
spelling pubmed-920342002-09-12 Nitrile Hydratase and Amidase from Rhodococcus rhodochrous Hydrolyze Acrylic Fibers and Granular Polyacrylonitriles Tauber, M. M. Cavaco-Paulo, A. Robra, K.-H. Gübitz, G. M. Appl Environ Microbiol Enzymology and Protein Engineering Rhodococcus rhodochrous NCIMB 11216 produced nitrile hydratase (320 nkat mg of protein(−1)) and amidase activity (38.4 nkat mg of protein(−1)) when grown on a medium containing propionitrile. These enzymes were able to hydrolyze nitrile groups of both granular polyacrylonitriles (PAN) and acrylic fibers. Nitrile groups of PAN40 (molecular mass, 40 kDa) and PAN190 (molecular mass, 190 kDa) were converted into the corresponding carbonic acids to 1.8 and 1.0%, respectively. In contrast, surfacial nitrile groups of acrylic fibers were only converted to the corresponding amides. X-ray photoelectron spectroscopy analysis showed that 16% of the surfacial nitrile groups were hydrolyzed by the R. rhodochrous enzymes. Due to the enzymatic modification, the acrylic fibers became more hydrophilic and thus, adsorption of dyes was enhanced. This was indicated by a 15% increase in the staining level (K/S value) for C.I. Basic Blue 9. American Society for Microbiology 2000-04 /pmc/articles/PMC92034/ /pubmed/10742253 Text en Copyright © 2000, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Enzymology and Protein Engineering
spellingShingle Enzymology and Protein Engineering
Tauber, M. M.
Cavaco-Paulo, A.
Robra, K.-H.
Gübitz, G. M.
Nitrile Hydratase and Amidase from Rhodococcus rhodochrous Hydrolyze Acrylic Fibers and Granular Polyacrylonitriles
description Rhodococcus rhodochrous NCIMB 11216 produced nitrile hydratase (320 nkat mg of protein(−1)) and amidase activity (38.4 nkat mg of protein(−1)) when grown on a medium containing propionitrile. These enzymes were able to hydrolyze nitrile groups of both granular polyacrylonitriles (PAN) and acrylic fibers. Nitrile groups of PAN40 (molecular mass, 40 kDa) and PAN190 (molecular mass, 190 kDa) were converted into the corresponding carbonic acids to 1.8 and 1.0%, respectively. In contrast, surfacial nitrile groups of acrylic fibers were only converted to the corresponding amides. X-ray photoelectron spectroscopy analysis showed that 16% of the surfacial nitrile groups were hydrolyzed by the R. rhodochrous enzymes. Due to the enzymatic modification, the acrylic fibers became more hydrophilic and thus, adsorption of dyes was enhanced. This was indicated by a 15% increase in the staining level (K/S value) for C.I. Basic Blue 9.
author Tauber, M. M.
Cavaco-Paulo, A.
Robra, K.-H.
Gübitz, G. M.
author_facet Tauber, M. M.
Cavaco-Paulo, A.
Robra, K.-H.
Gübitz, G. M.
author_sort Tauber, M. M.
title Nitrile Hydratase and Amidase from Rhodococcus rhodochrous Hydrolyze Acrylic Fibers and Granular Polyacrylonitriles
title_short Nitrile Hydratase and Amidase from Rhodococcus rhodochrous Hydrolyze Acrylic Fibers and Granular Polyacrylonitriles
title_full Nitrile Hydratase and Amidase from Rhodococcus rhodochrous Hydrolyze Acrylic Fibers and Granular Polyacrylonitriles
title_fullStr Nitrile Hydratase and Amidase from Rhodococcus rhodochrous Hydrolyze Acrylic Fibers and Granular Polyacrylonitriles
title_full_unstemmed Nitrile Hydratase and Amidase from Rhodococcus rhodochrous Hydrolyze Acrylic Fibers and Granular Polyacrylonitriles
title_sort nitrile hydratase and amidase from rhodococcus rhodochrous hydrolyze acrylic fibers and granular polyacrylonitriles
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 2000
url https://ncbi.nlm.nih.gov/pmc/articles/PMC92034/
https://ncbi.nlm.nih.gov/pubmed/10742253
_version_ 1759029656965611520

Registos relacionados