Genotypic Heterogeneity of Streptococcus oralis and Distinct Aciduric Subpopulations in Human Dental Plaque

The genotypic heterogeneity of Streptococcus oralis isolated from the oral cavity was investigated using repetitive extragenic palindromic PCR. Unrelated subjects harbored unique genotypes, with numerous genotypes being isolated from an individual. S. oralis is the predominant aciduric bacterium iso...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Alam, S., Brailsford, S. R., Adams, S., Allison, C., Sheehy, E., Zoitopoulos, L., Kidd, E. A., Beighton, D.
Formato: Artigo
Idioma:en
Publicado em: American Society for Microbiology 2000
Assuntos:
Microbial Ecology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC92151/
https://ncbi.nlm.nih.gov/pubmed/10919787
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
id pubmed-92151
record_format dspace
spelling pubmed-921512002-09-12 Genotypic Heterogeneity of Streptococcus oralis and Distinct Aciduric Subpopulations in Human Dental Plaque Alam, S. Brailsford, S. R. Adams, S. Allison, C. Sheehy, E. Zoitopoulos, L. Kidd, E. A. Beighton, D. Appl Environ Microbiol Microbial Ecology The genotypic heterogeneity of Streptococcus oralis isolated from the oral cavity was investigated using repetitive extragenic palindromic PCR. Unrelated subjects harbored unique genotypes, with numerous genotypes being isolated from an individual. S. oralis is the predominant aciduric bacterium isolated from noncarious tooth sites. Genotypic comparison of the aciduric populations isolated at pH 5.2 with those isolated from mitis-salivarius agar (MSA) (pH 7.0) indicated that the aciduric populations were genotypically distinct in the majority of subjects (χ(2) = 13.09; P = 0.0031). Neither the aciduric nor the MSA-isolated strains were stable, with no strains isolated at baseline being isolated 4 or 12 weeks later in the majority of subjects. The basis of this instability is unknown but is similar to that reported for Streptococcus mitis. Examination of S. oralis strains isolated from cohabiting couples demonstrated that in three of five couples, genotypically identical strains were isolated from both partners and this was confirmed by using Salmonella enteritidis repetitive element PCR and enterobacterial PCR typing. These data provide further evidence of the physiological and genotypic heterogeneity of non-mutans streptococci. The demonstration of distinct aciduric populations of S. oralis implies that the role of these and other non-mutans streptococci in the caries process requires reevaluation. American Society for Microbiology 2000-08 /pmc/articles/PMC92151/ /pubmed/10919787 Text en Copyright © 2000, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Microbial Ecology
spellingShingle Microbial Ecology
Alam, S.
Brailsford, S. R.
Adams, S.
Allison, C.
Sheehy, E.
Zoitopoulos, L.
Kidd, E. A.
Beighton, D.
Genotypic Heterogeneity of Streptococcus oralis and Distinct Aciduric Subpopulations in Human Dental Plaque
description The genotypic heterogeneity of Streptococcus oralis isolated from the oral cavity was investigated using repetitive extragenic palindromic PCR. Unrelated subjects harbored unique genotypes, with numerous genotypes being isolated from an individual. S. oralis is the predominant aciduric bacterium isolated from noncarious tooth sites. Genotypic comparison of the aciduric populations isolated at pH 5.2 with those isolated from mitis-salivarius agar (MSA) (pH 7.0) indicated that the aciduric populations were genotypically distinct in the majority of subjects (χ(2) = 13.09; P = 0.0031). Neither the aciduric nor the MSA-isolated strains were stable, with no strains isolated at baseline being isolated 4 or 12 weeks later in the majority of subjects. The basis of this instability is unknown but is similar to that reported for Streptococcus mitis. Examination of S. oralis strains isolated from cohabiting couples demonstrated that in three of five couples, genotypically identical strains were isolated from both partners and this was confirmed by using Salmonella enteritidis repetitive element PCR and enterobacterial PCR typing. These data provide further evidence of the physiological and genotypic heterogeneity of non-mutans streptococci. The demonstration of distinct aciduric populations of S. oralis implies that the role of these and other non-mutans streptococci in the caries process requires reevaluation.
author Alam, S.
Brailsford, S. R.
Adams, S.
Allison, C.
Sheehy, E.
Zoitopoulos, L.
Kidd, E. A.
Beighton, D.
author_facet Alam, S.
Brailsford, S. R.
Adams, S.
Allison, C.
Sheehy, E.
Zoitopoulos, L.
Kidd, E. A.
Beighton, D.
author_sort Alam, S.
title Genotypic Heterogeneity of Streptococcus oralis and Distinct Aciduric Subpopulations in Human Dental Plaque
title_short Genotypic Heterogeneity of Streptococcus oralis and Distinct Aciduric Subpopulations in Human Dental Plaque
title_full Genotypic Heterogeneity of Streptococcus oralis and Distinct Aciduric Subpopulations in Human Dental Plaque
title_fullStr Genotypic Heterogeneity of Streptococcus oralis and Distinct Aciduric Subpopulations in Human Dental Plaque
title_full_unstemmed Genotypic Heterogeneity of Streptococcus oralis and Distinct Aciduric Subpopulations in Human Dental Plaque
title_sort genotypic heterogeneity of streptococcus oralis and distinct aciduric subpopulations in human dental plaque
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 2000
url https://ncbi.nlm.nih.gov/pmc/articles/PMC92151/
https://ncbi.nlm.nih.gov/pubmed/10919787
_version_ 1759029673873899520

Registos relacionados