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Experimental Evolution of Enzyme Temperature Activity Profile: Selection In Vivo and Characterization of Low-Temperature-Adapted Mutants of Pyrococcus furiosus Ornithine Carbamoyltransferase
We have obtained mutants of Pyrococcus furiosus ornithine carbamoyltransferase active at low temperatures by selecting for complementation of an appropriate yeast mutant after in vivo mutagenesis. The mutants were double ones, still complementing at 15°C, a temperature already in the psychrophilic r...
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| Main Authors: | , , , |
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| 格式: | Artigo |
| 語言: | en |
| 出版: |
American Society for Microbiology
2001
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC94980/ https://ncbi.nlm.nih.gov/pubmed/11208811 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.3.1101-1105.2001 |
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