Gene Replacement Analysis of the Butyrolactone Autoregulator Receptor (FarA) Reveals that FarA Acts as a Novel Regulator in Secondary Metabolism of Streptomyces lavendulae FRI-5

IM-2 [(2R,3R,1′R)-2-1′-hydroxybutyl-3-hydroxymethyl γ-butanolide] is a γ-butyrolactone autoregulator which, in Streptomyces lavendulae FRI-5, switches off the production of d-cycloserine but switches on the production of a blue pigment and several nucleoside antibiotics. To clarify the in vivo funct...

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محفوظ في:
التفاصيل البيبلوغرافية
المؤلفون الرئيسيون: Kitani, Shigeru, Yamada, Yasuhiro, Nihira, Takuya
التنسيق: مقال
اللغة:en
منشور في: American Society for Microbiology 2001
الموضوعات:
Genetics and Molecular Biology
الوصول للمادة أونلاين:https://ncbi.nlm.nih.gov/pmc/articles/PMC95326/
https://ncbi.nlm.nih.gov/pubmed/11418577
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.14.4357-4363.2001
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spelling pubmed-953262002-09-13 Gene Replacement Analysis of the Butyrolactone Autoregulator Receptor (FarA) Reveals that FarA Acts as a Novel Regulator in Secondary Metabolism of Streptomyces lavendulae FRI-5 Kitani, Shigeru Yamada, Yasuhiro Nihira, Takuya J Bacteriol Genetics and Molecular Biology IM-2 [(2R,3R,1′R)-2-1′-hydroxybutyl-3-hydroxymethyl γ-butanolide] is a γ-butyrolactone autoregulator which, in Streptomyces lavendulae FRI-5, switches off the production of d-cycloserine but switches on the production of a blue pigment and several nucleoside antibiotics. To clarify the in vivo function of an IM-2-specific receptor (FarA) in the IM-2 signaling cascade of S. lavendulae FRI-5, a farA deletion mutant was constructed by means of homologous recombination. On several solid media, no significant difference in morphology was observed between the wild-type strain and the farA mutant (strain K104), which demonstrated that the IM-2–FarA system does not participate in the morphological control of S. lavendulae FRI-5. In liquid media, the farA mutant overproduced nucleoside antibiotics and produced blue pigment earlier than did the wild-type strain, suggesting that the FarA protein acts primarily as a negative regulator on the biosynthesis of these compounds in the absence of IM-2. However, contrary to the IM-2-dependent suppression of d-cycloserine production in the wild-type strain, overproduction of d-cycloserine was observed in the farA mutant, indicating for the first time that the presence of both IM-2 and intact FarA are necessary for the suppression of d-cycloserine biosynthesis. American Society for Microbiology 2001-07 /pmc/articles/PMC95326/ /pubmed/11418577 http://dx.doi.org/10.1128/JB.183.14.4357-4363.2001 Text en Copyright © 2001, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Genetics and Molecular Biology
spellingShingle Genetics and Molecular Biology
Kitani, Shigeru
Yamada, Yasuhiro
Nihira, Takuya
Gene Replacement Analysis of the Butyrolactone Autoregulator Receptor (FarA) Reveals that FarA Acts as a Novel Regulator in Secondary Metabolism of Streptomyces lavendulae FRI-5
description IM-2 [(2R,3R,1′R)-2-1′-hydroxybutyl-3-hydroxymethyl γ-butanolide] is a γ-butyrolactone autoregulator which, in Streptomyces lavendulae FRI-5, switches off the production of d-cycloserine but switches on the production of a blue pigment and several nucleoside antibiotics. To clarify the in vivo function of an IM-2-specific receptor (FarA) in the IM-2 signaling cascade of S. lavendulae FRI-5, a farA deletion mutant was constructed by means of homologous recombination. On several solid media, no significant difference in morphology was observed between the wild-type strain and the farA mutant (strain K104), which demonstrated that the IM-2–FarA system does not participate in the morphological control of S. lavendulae FRI-5. In liquid media, the farA mutant overproduced nucleoside antibiotics and produced blue pigment earlier than did the wild-type strain, suggesting that the FarA protein acts primarily as a negative regulator on the biosynthesis of these compounds in the absence of IM-2. However, contrary to the IM-2-dependent suppression of d-cycloserine production in the wild-type strain, overproduction of d-cycloserine was observed in the farA mutant, indicating for the first time that the presence of both IM-2 and intact FarA are necessary for the suppression of d-cycloserine biosynthesis.
author Kitani, Shigeru
Yamada, Yasuhiro
Nihira, Takuya
author_facet Kitani, Shigeru
Yamada, Yasuhiro
Nihira, Takuya
author_sort Kitani, Shigeru
title Gene Replacement Analysis of the Butyrolactone Autoregulator Receptor (FarA) Reveals that FarA Acts as a Novel Regulator in Secondary Metabolism of Streptomyces lavendulae FRI-5
title_short Gene Replacement Analysis of the Butyrolactone Autoregulator Receptor (FarA) Reveals that FarA Acts as a Novel Regulator in Secondary Metabolism of Streptomyces lavendulae FRI-5
title_full Gene Replacement Analysis of the Butyrolactone Autoregulator Receptor (FarA) Reveals that FarA Acts as a Novel Regulator in Secondary Metabolism of Streptomyces lavendulae FRI-5
title_fullStr Gene Replacement Analysis of the Butyrolactone Autoregulator Receptor (FarA) Reveals that FarA Acts as a Novel Regulator in Secondary Metabolism of Streptomyces lavendulae FRI-5
title_full_unstemmed Gene Replacement Analysis of the Butyrolactone Autoregulator Receptor (FarA) Reveals that FarA Acts as a Novel Regulator in Secondary Metabolism of Streptomyces lavendulae FRI-5
title_sort gene replacement analysis of the butyrolactone autoregulator receptor (fara) reveals that fara acts as a novel regulator in secondary metabolism of streptomyces lavendulae fri-5
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 2001
url https://ncbi.nlm.nih.gov/pmc/articles/PMC95326/
https://ncbi.nlm.nih.gov/pubmed/11418577
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.14.4357-4363.2001
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