Immune Response to Yersinia Outer Proteins and Other Yersinia pestis Antigens after Experimental Plague Infection in Mice

There is limited information concerning the nature and extent of the immune response to the virulence determinants of Yersinia pestis during the course of plague infection. In this study, we evaluated the humoral immune response of mice that survived lethal Y. pestis aerosol challenge after antibiot...

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Main Authors: Benner, Gretchen E., Andrews, Gerard P., Byrne, W. Russell, Strachan, Susan D., Sample, Allen K., Heath, David G., Friedlander, Arthur M.
Formato: Artigo
Idioma:en
Publicado em: American Society for Microbiology 1999
Assuntos:
Microbial Immunity and Vaccines
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC96547/
https://ncbi.nlm.nih.gov/pubmed/10085037
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spelling pubmed-965472002-09-12 Immune Response to Yersinia Outer Proteins and Other Yersinia pestis Antigens after Experimental Plague Infection in Mice Benner, Gretchen E. Andrews, Gerard P. Byrne, W. Russell Strachan, Susan D. Sample, Allen K. Heath, David G. Friedlander, Arthur M. Infect Immun Microbial Immunity and Vaccines There is limited information concerning the nature and extent of the immune response to the virulence determinants of Yersinia pestis during the course of plague infection. In this study, we evaluated the humoral immune response of mice that survived lethal Y. pestis aerosol challenge after antibiotic treatment. Such a model may replicate the clinical situation in humans and indicate which virulence determinants are expressed in vivo. Immunoglobulin G enzyme-linked immunosorbent assay and immunoblotting were performed by using purified, recombinant antigens including F1, V antigen, YpkA, YopH, YopM, YopB, YopD, YopN, YopE, YopK, plasminogen activator protease (Pla), and pH 6 antigen as well as purified lipopolysaccharide. The major antigens recognized by murine convalescent sera were F1, V antigen, YopH, YopM, YopD, and Pla. Early treatment with antibiotics tended to reduce the immune response and differences between antibiotic treatment regimens were noted. These results may indicate that only some virulence factors are expressed and/or immunogenic during infection. This information may prove useful for selecting potential vaccine candidates and for developing improved serologic diagnostic assays. American Society for Microbiology 1999-04 /pmc/articles/PMC96547/ /pubmed/10085037 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Microbial Immunity and Vaccines
spellingShingle Microbial Immunity and Vaccines
Benner, Gretchen E.
Andrews, Gerard P.
Byrne, W. Russell
Strachan, Susan D.
Sample, Allen K.
Heath, David G.
Friedlander, Arthur M.
Immune Response to Yersinia Outer Proteins and Other Yersinia pestis Antigens after Experimental Plague Infection in Mice
description There is limited information concerning the nature and extent of the immune response to the virulence determinants of Yersinia pestis during the course of plague infection. In this study, we evaluated the humoral immune response of mice that survived lethal Y. pestis aerosol challenge after antibiotic treatment. Such a model may replicate the clinical situation in humans and indicate which virulence determinants are expressed in vivo. Immunoglobulin G enzyme-linked immunosorbent assay and immunoblotting were performed by using purified, recombinant antigens including F1, V antigen, YpkA, YopH, YopM, YopB, YopD, YopN, YopE, YopK, plasminogen activator protease (Pla), and pH 6 antigen as well as purified lipopolysaccharide. The major antigens recognized by murine convalescent sera were F1, V antigen, YopH, YopM, YopD, and Pla. Early treatment with antibiotics tended to reduce the immune response and differences between antibiotic treatment regimens were noted. These results may indicate that only some virulence factors are expressed and/or immunogenic during infection. This information may prove useful for selecting potential vaccine candidates and for developing improved serologic diagnostic assays.
author Benner, Gretchen E.
Andrews, Gerard P.
Byrne, W. Russell
Strachan, Susan D.
Sample, Allen K.
Heath, David G.
Friedlander, Arthur M.
author_facet Benner, Gretchen E.
Andrews, Gerard P.
Byrne, W. Russell
Strachan, Susan D.
Sample, Allen K.
Heath, David G.
Friedlander, Arthur M.
author_sort Benner, Gretchen E.
title Immune Response to Yersinia Outer Proteins and Other Yersinia pestis Antigens after Experimental Plague Infection in Mice
title_short Immune Response to Yersinia Outer Proteins and Other Yersinia pestis Antigens after Experimental Plague Infection in Mice
title_full Immune Response to Yersinia Outer Proteins and Other Yersinia pestis Antigens after Experimental Plague Infection in Mice
title_fullStr Immune Response to Yersinia Outer Proteins and Other Yersinia pestis Antigens after Experimental Plague Infection in Mice
title_full_unstemmed Immune Response to Yersinia Outer Proteins and Other Yersinia pestis Antigens after Experimental Plague Infection in Mice
title_sort immune response to yersinia outer proteins and other yersinia pestis antigens after experimental plague infection in mice
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 1999
url https://ncbi.nlm.nih.gov/pmc/articles/PMC96547/
https://ncbi.nlm.nih.gov/pubmed/10085037
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