Drastic Reduction of a Filarial Infection in Eosinophilic Interleukin-5 Transgenic Mice

In order to establish the role of eosinophils in destroying parasites, transgenic mice have been used in experimental helminthiases but not in filariasis. Litomosoides sigmodontis offers a good opportunity for this study because it is the only filarial species that completes its life cycle in mice....

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Main Authors: Martin, Coralie, Le Goff, Laëtitia, Ungeheuer, Marie-Noelle, Vuong, Phat N., Bain, Odile
Formato: Artigo
Idioma:en
Publicado em: American Society for Microbiology 2000
Assuntos:
Fungal and Parasitic Infections
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC97655/
https://ncbi.nlm.nih.gov/pubmed/10816524
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spelling pubmed-976552002-09-12 Drastic Reduction of a Filarial Infection in Eosinophilic Interleukin-5 Transgenic Mice Martin, Coralie Le Goff, Laëtitia Ungeheuer, Marie-Noelle Vuong, Phat N. Bain, Odile Infect Immun Fungal and Parasitic Infections In order to establish the role of eosinophils in destroying parasites, transgenic mice have been used in experimental helminthiases but not in filariasis. Litomosoides sigmodontis offers a good opportunity for this study because it is the only filarial species that completes its life cycle in mice. Its development was compared in transgenic CBA/Ca mice overexpressing interleukin-5 (IL-5) and in wild-type mice following subcutaneous inoculation of 40 infective larvae. An acceleration of larval growth was observed in the IL-5 transgenic mice. However, the recovery rate of adult worms was considerably reduced in these mice, as evidenced 2 months postinoculation (p.i.). The reduction occurs between days 10 and 30 p.i. in the coelomic cavities. As early as day 10, spherical aggregates of eosinophils and macrophages are seen attached on live developing larvae (always similarly localized on the worm) in both wild-type and transgenic mice. However, on day 60 p.i., granulomas were found in the transgenic mice only, probably because of the higher density of eosinophils. Furthermore, on day 30 p.i., young filariae are seen trapped in granulomas, some of them surrounded by Splendore-Hoeppli deposits, which illustrates the release of the major basic protein by eosinophils. The high protection rate obtained (65%) is similar to that observed previously in BALB/c mice following vaccination with irradiated larvae. Both protocols have a common factor, the high production of IL-5 and eosinophilia. However, protection occurs later in primary infected transgenic mice because specific antibodies are not yet present at the time of challenge. American Society for Microbiology 2000-06 /pmc/articles/PMC97655/ /pubmed/10816524 Text en Copyright © 2000, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Fungal and Parasitic Infections
spellingShingle Fungal and Parasitic Infections
Martin, Coralie
Le Goff, Laëtitia
Ungeheuer, Marie-Noelle
Vuong, Phat N.
Bain, Odile
Drastic Reduction of a Filarial Infection in Eosinophilic Interleukin-5 Transgenic Mice
description In order to establish the role of eosinophils in destroying parasites, transgenic mice have been used in experimental helminthiases but not in filariasis. Litomosoides sigmodontis offers a good opportunity for this study because it is the only filarial species that completes its life cycle in mice. Its development was compared in transgenic CBA/Ca mice overexpressing interleukin-5 (IL-5) and in wild-type mice following subcutaneous inoculation of 40 infective larvae. An acceleration of larval growth was observed in the IL-5 transgenic mice. However, the recovery rate of adult worms was considerably reduced in these mice, as evidenced 2 months postinoculation (p.i.). The reduction occurs between days 10 and 30 p.i. in the coelomic cavities. As early as day 10, spherical aggregates of eosinophils and macrophages are seen attached on live developing larvae (always similarly localized on the worm) in both wild-type and transgenic mice. However, on day 60 p.i., granulomas were found in the transgenic mice only, probably because of the higher density of eosinophils. Furthermore, on day 30 p.i., young filariae are seen trapped in granulomas, some of them surrounded by Splendore-Hoeppli deposits, which illustrates the release of the major basic protein by eosinophils. The high protection rate obtained (65%) is similar to that observed previously in BALB/c mice following vaccination with irradiated larvae. Both protocols have a common factor, the high production of IL-5 and eosinophilia. However, protection occurs later in primary infected transgenic mice because specific antibodies are not yet present at the time of challenge.
author Martin, Coralie
Le Goff, Laëtitia
Ungeheuer, Marie-Noelle
Vuong, Phat N.
Bain, Odile
author_facet Martin, Coralie
Le Goff, Laëtitia
Ungeheuer, Marie-Noelle
Vuong, Phat N.
Bain, Odile
author_sort Martin, Coralie
title Drastic Reduction of a Filarial Infection in Eosinophilic Interleukin-5 Transgenic Mice
title_short Drastic Reduction of a Filarial Infection in Eosinophilic Interleukin-5 Transgenic Mice
title_full Drastic Reduction of a Filarial Infection in Eosinophilic Interleukin-5 Transgenic Mice
title_fullStr Drastic Reduction of a Filarial Infection in Eosinophilic Interleukin-5 Transgenic Mice
title_full_unstemmed Drastic Reduction of a Filarial Infection in Eosinophilic Interleukin-5 Transgenic Mice
title_sort drastic reduction of a filarial infection in eosinophilic interleukin-5 transgenic mice
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 2000
url https://ncbi.nlm.nih.gov/pmc/articles/PMC97655/
https://ncbi.nlm.nih.gov/pubmed/10816524
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