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High-throughput genome editing and phenotyping facilitated by high resolution melting curve analysis.

With the goal to generate and characterize the phenotypes of null alleles in all genes within an organism and the recent advances in custom nucleases, genome editing limitations have moved from mutation generation to mutation detection. We previously demonstrated that High Resolution Melting (HRM) a...

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Bibliografske podrobnosti
izdano v:PLoS ONE
Main Authors: Holly R Thomas, Stefanie M Percival, Bradley K Yoder, John M Parant
Format: Artigo
Izdano: Public Library of Science (PLoS) 2014-01-01
Online dostop:http://europepmc.org/articles/PMC4263700?pdf=render
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