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Construction of A High-efficiency Homologous Recombination and Uridine/Uracil Auxotroph Strain of Aspergillus oryzae RIB40

Objective: An auxotrophic strain with high efficiency of homologous recombination was developed in Aspergillus oryzae RIB40. Methods: The AopyrG mutant of A. oryzae RIB40 was firstly obtained using a homologous recombination technique and the 5-fluoroorotic acid (5-FOA) selection for the uridine/ura...

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Bibliografiske detaljer
Udgivet i:Shipin gongye ke-ji
Main Authors: Yuqing GAO, Haojie ZHANG, Danfeng ZHANG, Yutian PAN, Feng ZHANG
Format: Artigo
Udgivet: The editorial department of Science and Technology of Food Industry 2023-01-01
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Online adgang:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022040179
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