Patch-clamp detection of macromolecular translocation along nuclear pores

The present paper reviews the application of patch-clamp principles to the detection and measurement of macromolecular translocation along the nuclear pores. We demonstrate that the tight-seal 'gigaseal' between the pipette tip and the nuclear membrane is possible in the presence of fully...

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Détails bibliographiques
Publié dans:Brazilian Journal of Medical and Biological Research Brazilian Journal of Medical and Biological Research, Volume: 31, Issue: 3, Pages: 333-354, Published: MAR 1998
Auteurs principaux: Bustamante, J.O., Varanda, W.A.
Format: Article
Langue:English
Publié: Associação Brasileira de Divulgação Científica 1998
Sujets:
Nuclear pores
Nuclear envelope
Cell nucleus
Nucleocytoplasmic transport
Macromolecular transport
Gene activity
Gene expression
Transcriptional control
Ion channels
Patch-clamp
Measurement
Accès en ligne:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300003&lng=en&tlng=en
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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300003&lng=en&tlng=en

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