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Use of the Duplex TaqMan PCR System for Detection of Shiga-Like Toxin-Producing Escherichia coli O157
Real-time PCR assays have been applied for the detection and quantification of pathogens in recent years. In this study two combinations of primers and fluorescent probes were designed according to the sequences of the rfb(Escherichia coli O157) and stx(2) genes. Analysis of 217 bacterial strains de...
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| Format: | Artikel |
| Sprache: | English |
| Veröffentlicht: |
American Society for Microbiology
2005
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1151888/ https://ncbi.nlm.nih.gov/pubmed/15956382 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.43.6.2668-2673.2005 |
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