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Use of a novel rapid preparation of fat-cell plasma membranes employing Percoll to investigate the effects of insulin and adrenaline on membrane protein phosphorylation within intact fat-cells.
1. A rapid method was developed for the preparation of plasma membranes from either isolated rat fat-cells or intact epididymal fat-pads with the use of density-gradient centrifugation in the presence of Percoll. On the basis of 5'-nucleotidase activity, the yield of plasma membranes was about...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | English |
| Publicado em: |
1980
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1162360/ https://ncbi.nlm.nih.gov/pubmed/6263255 |
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