Conjugation of Lentivirus to Paramagnetic Particles via Nonviral Proteins Allows Efficient Concentration and Infection of Primary Acute Myeloid Leukemia Cells

Nonviral producer cell proteins incorporated into retroviral vector surfaces profoundly influence infectivity and in vivo half-life. We report the purification and concentration of lentiviral vectors using these surface proteins as an efficient gene transduction strategy. Biotinylation of these prot...

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Detalhes bibliográficos
Main Authors: Chan, Lucas, Nesbeth, Darren, MacKey, Taylor, Galea-Lauri, Joanna, Gäken, Joop, Martin, Francisco, Collins, Mary, Mufti, Ghulam, Farzaneh, Farzin, Darling, David
Formato: Artigo
Idioma:English
Publicado em: American Society for Microbiology 2005
Assuntos:
Gene Delivery
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1235865/
https://ncbi.nlm.nih.gov/pubmed/16189021
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.79.20.13190-13194.2005
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https://ncbi.nlm.nih.gov/pmc/articles/PMC1235865/
https://ncbi.nlm.nih.gov/pubmed/16189021
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.79.20.13190-13194.2005

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