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Mg2+ binding and structural stability of mature and in vitro synthesized unmodified Escherichia coli tRNAPhe.

Mature tRNAPhe from Escherichia coli and the transcript of its gene lacking modified nucleotides were compared by a variety of physical techniques. Melting experiments revealed that at a low Mg2+level the transcript was partially denatured, while the mature tRNA possessed intact tertiary interaction...

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Detalhes bibliográficos
Main Authors: Serebrov, V, Vassilenko, K, Kholod, N, Gross, H J, Kisselev, L
Formato: Artigo
Idioma:en
Publicado em: 1998
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC147623/
https://ncbi.nlm.nih.gov/pubmed/9592160
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