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Sequence-specific RNase H cleavage of gag mRNA from HIV-1 infected cells by an antisense oligonucleotide in vitro.

We have used a ribonuclease protection assay to investigate RNase H cleavage of HIV-1 mRNA mediated by phosphorothioate antisense oligonucleotides complementary to the gag region of the HIV-1 genome in vitro. Cell lysate experiments in H9 and U937 cells chronically infected with HIV-1 IIIB showed RN...

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Main Authors:	Veal, G J, Agrawal, S, Byrn, R A, Khrapko, K, Coller, H A, Hanekamp, J S, Thilly, W G, Fujiwara, J, Oishi, M, Ramos, W, Liu, G, Giroux, C N, Tomkinson, A E, Tomaszewski, R, Mogielnicka, E, Jerzmanowski, A, Pan, B, Mitra, S N, Sun, L, Hart, D, Sundaralingam, M, Grünenfelder, Björn, Gehrig, Stefanie, Jenal, Urs, Marcus, Yehouda, Altman-Gueta, Hagit, Finkler, Aliza, Gurevitz, Michael, Vergauwen, Bjorn, Pauwels, Frederik, Vaneechoutte, Mario, Van Beeumen, Jozef J., Solem, Christian, Koebmann, Brian J., Jensen, Peter R., Mohri, Yoshio, Goto, Simon, Nakahigashi, Kenji, Inokuchi, Hachiro, Stewart, Philip S., Starčič, Marjanca, Žgur-Bertok, Darja, Jordi, Bart J. A. M., Wösten, Marc M. S. M., Gaastra, Wim, van Putten, Jos P. M., Tun-Garrido, Cristina, Bustos, Patricia, González, Víctor, Brom, Susana, Kanai, Tamotsu, Ito, Sota, Imanaka, Tadayuki, O'Toole, Ronan, Smeulders, Marjan J., Blokpoel, Marian C., Kay, Emily J., Lougheed, Kathryn, Williams, Huw D., Martinussen, Jan, Wadskov-Hansen, Steen L. L., Hammer, Karin, Kuhnert, Wendi L., Quivey, Jr., Robert G., Ng, Wing-On, Grossman, Arthur R., Bhaya, Devaki, Wyrzykowski, Jennifer, Volkert, Michael R., Pérez-Pantoja, D., Ledger, T., Pieper, D. H., González, B., Hihara, Yukako, Sonoike, Kintake, Kanehisa, Minoru, Ikeuchi, Masahiko, von Wallbrunn, Angelika, Richnow, Hans Hermann, Neumann, Grit, Meinhardt, Friedhelm, Heipieper, Hermann J., Murashima, Koichiro, Kosugi, Akihiko, Doi, Roy H., Vattanaviboon, Paiboon, Whangsuk, Wirongrong, Mongkolsuk, Skorn, Silversmith, Ruth E., Guanga, Gerald P., Betts, Laurie, Chu, Carolyn, Zhao, Rui, Bourret, Robert B., Frirdich, Emilisa, Lindner, Buko, Holst, Otto, Whitfield, Chris, Zimmermann, Rainer, Kempf, Volkhard A. J., Schiltz, Emile, Oberle, Karin, Sander, Anna, Hilbert, David W., Piggot, Patrick J., Laatu, Minna, Condemine, Guy, Moriya, Tatsuki, Nakane, Akitaka, Yanai, Koji, Sumida, Naomi, Koga, Jinichiro, Murakami, Takeshi, Kono, Toshiaki, Sylvestre, Patricia, Couture-Tosi, Evelyne, Mock, Michèle, Rybniker, Jan, Wolke, Martina, Haefs, Christiane, Plum, Georg, Di Guilmi, Anne Marie, Dessen, Andréa, Dideberg, Otto, Vernet, Thierry, Zhao, Li, Kanamaru, Shuji, Chaidirek, Chatree'chalerm, Arisaka, Fumio, Hirai, Teruhisa, Heymann, Jürgen A. W., Maloney, Peter C., Subramaniam, Sriram, Jordan, Sean W., Cronan, Jr., John E., Kim, Hyun-Jin, Kim, Sam-In, Ratnayake-Lecamwasam, Manoja, Tachikawa, Kiyoshi, Sonenshein, Abraham L., Strauch, Mark, Izquierdo, Luis, Merino, Susana, Regué, Miguel, Rodriguez, Florencia, Tomás, Juan M., Fink, Doran L., St. Geme III, Joseph W., Parkinson, John S., Burks, C, Discala, C, Ninnin, M, Achard, F, Barillot, E, Vaysseix, G, Benson, D A, Boguski, M S, Lipman, D J, Ostell, J, Ouellette, B F, Rapp, B A, Wheeler, D L, Stoesser, G, Tuli, M A, Lopez, R, Sterk, P, Sugawara, H, Miyazaki, S, Gojobori, T, Tateno, Y, Ogata, H, Goto, S, Sato, K, Fujibuchi, W, Bono, H, Kanehisa, M, Skupski, M P, Booker, M, Farmer, A, Harpold, M, Huang, W, Inman, J, Kiphart, D, Kodira, C, Root, S, Schilkey, F, Schwertfeger, J, Siepel, A, Stamper, D, Thayer, N, Thompson, R, Wortman, J, Zhuang, J J, Harger, C, Barker, W C, Garavelli, J S, McGarvey, P B, Marzec, C R, Orcutt, B C, Srinivasarao, G Y, Yeh, L S, Ledley, R S, Mewes, H W, Pfeiffer, F, Tsugita, A, Wu, C, Heumann, K, Kaps, A, Mayer, K, Stocker, S, Frishman, D
Formato:	Artigo
Idioma:	English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English English
Publicado em:	American Society for Microbiology 1998
Assuntos:	Research Article Microbial Cell Biology Enzymes and Proteins Physiology and Metabolism Genomics and Proteomics Guest Commentaries Bacteriophages, Transposons, and Plasmids Microbial Communities and Interactions Signal Transduction Genetics and Molecular Biology Gene Regulation Molecular Biology of Pathogens Plant Microbiology Structural Biology
Acesso em linha:	https://ncbi.nlm.nih.gov/pmc/articles/PMC148044/ https://ncbi.nlm.nih.gov/pubmed/9837998 https://ncbi.nlm.nih.gov/pmc/articles/PMC148045/ https://ncbi.nlm.nih.gov/pubmed/9838007 https://ncbi.nlm.nih.gov/pmc/articles/PMC148046/ https://ncbi.nlm.nih.gov/pubmed/9838006 https://ncbi.nlm.nih.gov/pmc/articles/PMC148047/ https://ncbi.nlm.nih.gov/pubmed/9837999 https://ncbi.nlm.nih.gov/pmc/articles/PMC148048/ https://ncbi.nlm.nih.gov/pubmed/9837988 https://ncbi.nlm.nih.gov/pmc/articles/PMC148049/ https://ncbi.nlm.nih.gov/pubmed/9838002 https://ncbi.nlm.nih.gov/pmc/articles/PMC148050/ https://ncbi.nlm.nih.gov/pubmed/12591880 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.185.5.1624-1633.2003 https://ncbi.nlm.nih.gov/pmc/articles/PMC148051/ https://ncbi.nlm.nih.gov/pubmed/12591867 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.185.5.1509-1517.2003 https://ncbi.nlm.nih.gov/pmc/articles/PMC148052/ https://ncbi.nlm.nih.gov/pubmed/12591874 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Sequence-specific RNase H cleavage of gag mRNA from HIV-1 infected cells by an antisense oligonucleotide in vitro.

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