A Methyltransferase Targeting Assay Reveals Silencer-Telomere Interactions in Budding Yeast

We have designed a modified version of the Dam identification technique and used it to probe higher-order chromatin structure in Saccharomyces cerevisiae. We fused the bacterial DNA methyltransferase Dam to the DNA-binding domain of TetR and targeted the resulting chimera to Tet operators inserted i...

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Detalhes bibliográficos
Main Authors: Lebrun, Eleonore, Fourel, Geneviève, Defossez, Pierre-Antoine, Gilson, Eric
Formato: Artigo
Idioma:en
Publicado em: American Society for Microbiology 2003
Assuntos:
DNA Dynamics and Chromosome Structure
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC151690/
https://ncbi.nlm.nih.gov/pubmed/12588971
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MCB.23.5.1498-1508.2003
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https://ncbi.nlm.nih.gov/pmc/articles/PMC151690/
https://ncbi.nlm.nih.gov/pubmed/12588971
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MCB.23.5.1498-1508.2003

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