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Mammalian thioredoxin reductase: Oxidation of the C-terminal cysteine/selenocysteine active site forms a thioselenide, and replacement of selenium with sulfur markedly reduces catalytic activity

Mammalian cytosolic thioredoxin reductase (TrxR) has a redox center, consisting of Cys(59)/Cys(64) adjacent to the flavin ring of FAD and another center consisting of Cys(497)/selenocysteine (SeCys)(498) near the C terminus. We now show that the C-terminal Cys(497)-SH/SeCys(498)-Se(−) of NADPH-reduc...

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Detalhes bibliográficos
Main Authors: Lee, Seung-Rock, Bar-Noy, Shoshana, Kwon, Jaeyul, Levine, Rodney L., Stadtman, Thressa C., Rhee, Sue Goo
Formato: Artigo
Idioma:en
Publicado em: The National Academy of Sciences 2000
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC15961/
https://ncbi.nlm.nih.gov/pubmed/10688911
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