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A positive genetic selection for disrupting protein–protein interactions: Identification of CREB mutations that prevent association with the coactivator CBP
The Escherichia coli tet-repressor (TetR) operator system was used to develop a variation of the yeast two-hybrid assay in which disruptions of protein–protein interactions can be identified by a positive selection. This assay, designated the “split-hybrid system,” contains a two-component reporter....
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | en |
| Publicado em: |
The National Academy of Sciences of the USA
1996
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC19461/ https://ncbi.nlm.nih.gov/pubmed/8943032 |
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