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Generation of Tn5 insertions in streptococcal conjugative transposon Tn916.
A method has been developed for the introduction of Tn5 into Escherichia coli plasmid chimeras containing Streptococcus faecalis DNA. Tn5 could be introduced via a lambda::Tn5 delivery vehicle. The system proved to be particularly efficient and facilitated insertions at numerous sites on DNA contain...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | en |
| Udgivet: |
1987
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC203811/ https://ncbi.nlm.nih.gov/pubmed/3038014 |
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